Can cytoplasmic donation rescue aged oocytes?

Reproductive medicine and biology

article

Atsushi Tanaka¹  Seiji Watanabe² 

[1] Saint Mother Obstetrics and Gynecology Clinic and Institute for ART;Department of Anatomical Science, Hirosaki University Graduate School of Medicine

关键词: aged oocytes;    cytoplasmic donation;    germinal vesicle transfer (GVT);    mitochondrial DNA (mtDNA);    spindle chromosome transfer (ST);   

DOI  :  10.1002/rmb2.12252

学科分类：工业工程学

来源: Wiley

【 摘 要 】

Background: The pregnancy and delivery rates following assisted reproductive tech‐ nology (ART) start to decrease and that the miscarriage rate increases rapidly from 35 years old. The miscarriage rate exceeds 50% at 43 years old. The number of ane‐ uploid fetuses in miscarriages increases according to female age, reaching more than 90% when women are over 40 years old. Methods: Different cytoplasmic donation technologies used to rescue aged oocytes with high percentage of aneuploidy were analyzed, and their efficacy compared. Main findings (Results): Germinal vesicle transfer (GVT) might be superior to spindle chromosome transfer (ST) theoretically from the point of higher capability of rescu‐ ing the disjunction at meiosis I which cannot be helped by ST. However, actually, in vitro maturation (IVM) of oocyte after GVT has not yet been totally completed. ST among other nuclear donations showed the higher possibility to rescue them, due to the fact it does not require in vitro maturation and it has an ethical advantage over pronuclear transfer (PNT) which requires the destruction of an embryo. Conclusion: Spindle chromosome transfer has the potential to rescue aged oocytes to some extent, but we have to continue the basic study further to establish the clini‐ cal application of cytoplasmic donation to rescue aged oocytes.

【 授权许可】

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