Long noncoding RNA TUG1 regulates the progression of colorectal cancer through miR-542-3p/TRIB2 axis and Wnt/β-catenin pathway

Diagnostic Pathology

Xiaoran Guo¹  Wei Zhang¹  Linshan Luo¹  Quanlin Liu¹  Keshun Han²  Shue Wei³  Ruitao Liu³ 

[1] Department of Colorectal Surgery, Zhengzhou Anorectal Hospital, No. 51, Longhai East Road, 450004, Zhengzhou, China;Department of Constipation, Zhengzhou Anorectal Hospital, Zhengzhou, China;Department of Large Intestine, Zhengzhou Anorectal Hospital, Zhengzhou, China;

关键词: Colorectal cancer;    TUG1;    miR-542-3p;    TRIB2;    Wnt/β-catenin pathway;   

DOI  :  10.1186/s13000-021-01101-7

来源: Springer

【 摘 要 】

BackgroundColorectal cancer (CRC) is one of the third normal malignancy worldwide. Taurine-upregulated gene 1 (TUG1), a member of long noncoding RNAs (lncRNAs), has been reported to be involved in various cancers. However, the mechanism underlying TUG1 in the progression of CRC remains unclear.MethodsThe expression of TUG1, microRNA-542-3p (miR-542-3p), and tribbles homolog 2 (TRIB2) in CRC tissues and cells (LoVo and HCT116) were detected by quantitative real-time PCR (qRT-PCR). Methyl thiazolyl tetrazolium (MTT), transwell and flow cytometry assays were employed to evaluate the effects of TUG1 in CRC cells. The interaction between miR-542-3p and TUG1 or TRIB2 were verified by dual-luciferase reporter assay. A xenograft tumor model in nude mice was established to investigate the biological role of TUG1 in CRC in vivo.ResultsTUG1 was increased in CRC tissues and cells (LoVo and HCT116) in contrast with adjacent normal tissues and normal intestinal mucous cells (CCC-HIE-2). Downregulation of TUG1 or TRIB2 suppressed the proliferation, migration, invasion, and induced apoptosis in CRC cells. And knockdown of TUG1 repressed tumor growth in vivo. Besides, overexpression of TRIB2 reversed the effects of TUG1 depletion on the progression of CRC. Meanwhile, TUG1 interacted with miR-542-3p and TRIB2 was a target of miR-542-3p. Furthermore, miR-542-3p knockdown or TRIB2 overexpression partly reversed the suppression effect of TUG1 depletion on the Wnt/β-catenin pathway.ConclusionsTUG1 served as a tumor promoter, impeded the progression of CRC by miR-542-3p/TRIB2 axis to inactivate of Wnt/β-catenin pathway, which providing a novel target for CRC treatment.

【 授权许可】

CC BY