Electrospun fibrous sponge via short fiber for mimicking 3D ECM

Journal of Nanobiotechnology

Lei Wang¹  Juan Wang¹  Wenguo Cui¹  Liang Chen¹  Dejian Qian²  Yan Wang³  Yan Li⁴  Xiumei Mo⁵ 

[1] Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, 200025, Shanghai, People’s Republic of China;Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Key Laboratory of Rheumatic Disease and Translational Medical Shandong, 250014, Jinan, People’s Republic of China;Department of Plastic Surgery, Shandong Provincial Maternal and Child Health Care Hospital, Cheeloo College of Medicine, Shandong University, 250061, Shandong, People’s Republic of China;Department of Burn Surgery, Clinical Medicine, Weifang Medical University, 261053, Shandong, People’s Republic of China;Department of Plastic Surgery, Shandong Provincial Maternal and Child Health Care Hospital, Cheeloo College of Medicine, Shandong University, 250061, Shandong, People’s Republic of China;Department of Burn Surgery, Clinical Medicine, Weifang Medical University, 261053, Shandong, People’s Republic of China;Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, 200025, Shanghai, People’s Republic of China;State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 201620, Shanghai, China;

关键词: Extracellular matrix;    Electrospun;    Micro/nano fibers;    Sponges;    Tissue regeneration;   

DOI  :  10.1186/s12951-021-00878-5

来源: Springer

【 摘 要 】

BackgroundMost of the natural extracellular matrix (ECM) is a three-dimensional (3D) network structure of micro/nanofibers for cell adhesion and growth of 3D. Electrospun fibers distinctive mimicked 2D ECM, however, it is impossible to simulate 3D ECM because of longitudinal collapse of continuous micro/nanofibers. Herein, 3D electrospun micro/nano-fibrous sponge was fabricated via electrospinning, homogenization, shaping and thermal crosslinking for 3D tissue regeneration of cells and vascular.ResultsFibrous sponge exhibited high porosity, water absorption and compression resilience and no chemical crosslinked agent was used in preparation process. In vitro studies showed that the 3D short fiber sponge provided an oxygen-rich environment for cell growth, which was conducive to the 3D proliferation and growth of HUVECs, stimulated the expression of VEGF, and well promoted the vascularization of HUVECs. In vivo studies showed that the 3D short fiber sponges had a good 3D adhesion to the chronic wound of diabetes in rats. Furthermore, 3D short fibrous sponges were better than 2D micro/nanofiber membranes in promoting the repair of diabetic full-thickness skin defects including wound healing, hair follicle regeneration, angiogenesis, collagen secretion.ConclusionTherefore, electrospun short fibrous sponges are special candidates for mimicking the 3D ECM and promoting 3D regeneration of tissue.Graphic Abstract

【 授权许可】

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