TORC1 regulates the transcriptional response to glucose and developmental cycle via the Tap42-Sit4-Rrd1/2 pathway in Saccharomyces cerevisiae

BMC Biology

Corinna Jie Hui Goh¹  Quan Feng Sin¹  Jacqueline Lewis¹  Mohammad Alfatah¹  Wei Ting Lee¹  Jin Huei Wong¹  Yong Cheow Lee¹  Prakash Arumugam²  Shawn Hoon³  Vidhya Gomathi Krishnan³  Kiat Whye Kong³ 

[1] Bioinformatics Institute, 30 Biopolis Street, 138671, Singapore, Singapore;Bioinformatics Institute, 30 Biopolis Street, 138671, Singapore, Singapore;Singapore Institute for Food and Biotechnology Innovation, 31 Biopolis Way, #01-02 Nanos, 138669, Singapore, Singapore;School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore;Molecular Engineering Lab, 61 Biopolis Drive, 138673, Singapore, Singapore;

关键词: Transcriptional response to glucose;    TORC1;    Tap42/Sit4/Rrd1-2 module;    Spore germination;   

DOI  :  10.1186/s12915-021-01030-3

来源: Springer

【 摘 要 】

BackgroundTarget of Rapamycin Complex 1 (TORC1) is a highly conserved eukaryotic protein complex that couples the presence of growth factors and nutrients in the environment with cellular proliferation. TORC1 is primarily implicated in linking amino acid levels with cellular growth in yeast and mammals. Although glucose deprivation has been shown to cause TORC1 inactivation in yeast, the precise role of TORC1 in glucose signaling and the underlying mechanisms remain unclear.ResultsWe demonstrate that the presence of glucose in the growth medium is both necessary and sufficient for TORC1 activation. TORC1 activity increases upon addition of glucose to yeast cells growing in a non-fermentable carbon source. Conversely, shifting yeast cells from glucose to a non-fermentable carbon source reduces TORC1 activity. Analysis of transcriptomic data revealed that glucose and TORC1 co-regulate about 27% (1668/6004) of yeast genes. We demonstrate that TORC1 orchestrates the expression of glucose-responsive genes mainly via the Tap42-Sit4-Rrd1/2 pathway. To confirm TORC1’s function in glucose signaling, we tested its role in spore germination, a glucose-dependent developmental state transition in yeast. TORC1 regulates the glucose-responsive genes during spore germination and inhibition of TORC1 blocks spore germination.ConclusionsOur studies indicate that a regulatory loop that involves activation of TORC1 by glucose and regulation of glucose-responsive genes by TORC1, mediates nutritional control of growth and development in yeast.

【 授权许可】

CC BY