Discrepancy in p16 expression in patients with HPV-associated head and neck squamous cell carcinoma in Thailand: clinical characteristics and survival outcomes

BMC Cancer

Nuttapong Ngamphaiboon¹  Teerada Siripoon¹  Poompis Pattaranutaporn²  Duangjai Pangpunyakulchai³  Suda Sanpapant³  Sasithorn Foyhirun³  Lalida Arsa⁴  Artit Jinawath⁴  Natini Jinawath⁵  Narumol Trachu⁶ 

[1] Division of Medical Oncology, Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;Division of Radiation Oncology, Department of Radiology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;Immunohistopathology and Special Laboratory, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;Molecular Histopathology Laboratory, Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;Ramathibodi Comprehensive Cancer Center, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;Integrative Computational BioScience Center (ICBS), Mahidol University, Nakhon Pathom, Thailand;Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;Research center, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;

关键词: Head and neck squamous cell carcinoma;    p16;    HPV;    Oropharyngeal squamous cell carcinoma;    OPSCC;   

DOI  :  10.1186/s12885-021-08213-9

来源: Springer

【 摘 要 】

BackgroundLower prevalence HPV infection has been previously reported in Thai population when compared with Western countries. p16 expression indicates HPV-associated oropharyngeal squamous cell carcinoma (OPSCC), but not non-OPSCC. We therefore evaluated the characteristic and association of p16 and HPV in Thai patients with HNSCC.MethodsWe used immunohistochemistry and qPCR, respectively, to detect p16 and HPV DNA in archrival formalin-fixed paraffin-embedded HNSCC tissues. Patient characteristics and survival were analyzed.Resultsp16 expression was detected in tumors of 72 of 662 (10.9%) patients with HNSCC and was significantly associated with higher-grade histology, advanced nodal stage, and oropharynx. p16 was expressed in 28 and 6.5% of patients with OPSCC or non-OPSCC, respectively, and HPV DNA was detected in 15.6 and 1% of patients, respectively. Using p16 as a surrogate for HPV status, sensitivities were 80 and 25% in OPSCC and non-OPSCC, respectively. Positive and negative predictive rates of OPSCC were 38 and 95%. Discordance rates between HPV and p16 were 23 and 7% in OPSCC and non-OPSCC, respectively. Overall survival (OS) were significantly longer in both p16-positive OPSCC (p = 0.049), and non-OPSCC (p = 0.003).ConclusionsLow prevalence of p16 and HPV associated OPSCC and non-OPSCC were confirmed in Thai patients. High discordance and low positive predictive rates of p16 were observed in HPV-associated OPSCC. p16 was a significant prognostic factor for OS for patients with OPSCC or non-OPSCC. Therefore, HPV testing should be performed to assess the association of HPV with HNSCC regardless of p16 expression.

【 授权许可】

CC BY