Metabolome and transcriptome profiling reveals anthocyanin contents and anthocyanin-related genes of chimeric leaves in Ananas comosus var. bracteatus

BMC Genomics

Yehua He¹  Wei Yang²  Huiling Zhang²  Jiawen Liu²  Jiaheng Luo²  Xuzixin Zhou²  Xi Li²  Zhuo Huang²  Mark Owusu Adjei²  Yanbin Xue²  Hao Hu²  Lingxia Sun²  Jun Ma²  Meiqin Mao²  Lijun Feng² 

[1] College of Horticulture, South China Agricultural University, Guangzhou, China;College of Landscape Architecture, Sichuan Agricultural University, Chengdu, China;

关键词: Ananas comosus;    Anthocyanin;    CIELAB;    Metabolomics;    Transcriptomics;    MYB transcription factor;   

DOI  :  10.1186/s12864-021-07642-x

来源: Springer

【 摘 要 】

BackgroundAnanas comosus var. bracteatus is a colorful plant used as a cut flower or landscape ornamental. The unique foliage color of this plant includes both green and red leaves and, as a trait of interest, deserves investigation. In order to explore the pigments behind the red section of the chimeric leaves, the green and red parts of chimeric leaves of Ananas comosus var. bracteatus were sampled and analyzed at phenotypic, cellular and molecular levels in this study.ResultsThe CIELAB results indicated that the a* values and L* values samples had significant differences between two parts. Freehand sections showed that anthocyanin presented limited accumulation in the green leaf tissues but obviously accumulation in the epidermal cells of red tissues. Transcriptomic and metabolomic analyses were performed by RNA-seq and LC-ESI-MS/MS. Among the 508 identified metabolites, 10 kinds of anthocyanins were detected, with 6 significantly different between the two samples. The cyanidin-3,5-O-diglucoside content that accounts for nearly 95.6% in red samples was significantly higher than green samples. RNA-Seq analyses showed that 11 out of 40 anthocyanin-related genes were differentially expressed between the green and red samples. Transcriptome and metabolome correlations were determined by nine quadrant analyses, and 9 anthocyanin-related genes, including MYB5 and MYB82, were correlated with 7 anthocyanin-related metabolites in the third quadrant in which genes and metabolites showing consistent change. Particularly, the PCCs between these two MYB genes and cyanidin-3,5-O-diglucoside were above 0.95.ConclusionPhenotypic colors are closely related to the tissue structures of different leaf parts of Ananas comosus var. bracteatus, and two MYB transcription factors might contribute to differences of anthocyanin accumulation in two parts of Ananas comosus var. bracteatus chimeric leaves. This study lay a foundation for further researches on functions of MYBs in Ananas comosus var. bracteatus and provides new insights to anthocyanin accumulation in different parts of chimeric leaves.

【 授权许可】

CC BY