Increased dietary availability of selenium in rainbow trout (Oncorhynchus mykiss) improves its plasma antioxidant capacity and resistance to infection with Piscirickettsia salmonis

Veterinary Research

Jurij Wacyk¹  Francisca Vera-Tamargo²  Madelaine Mejías²  Ignacio Chávez-Báez²  Rodrigo Pulgar²  Liliana Montt²  Javiera Pérez-Valenzuela²  Dinka Mandakovic³  Daniela Ortiz⁴  Pablo Salgado⁴ 

[1] Center for Research and Innovation in Aquaculture (CRIA), Universidad de Chile, Santiago, Santiago, Chile;Laboratorio de Nutrición Animal (LABNA). Facultad de Ciencias Agronómicas, Producción Animal, Universidad de Chile, 11315, Santa Rosa, La Pintana, Chile;Laboratorio de Genómica y Genética de Interacciones Biológicas (LG2IB), Instituto de Nutrición y Tecnología de los Alimento, Universidad de Chile, El Líbano, Macul, 5524, Santiago, Chile;Center for Research and Innovation in Aquaculture (CRIA), Universidad de Chile, Santiago, Santiago, Chile;Laboratorio de Genómica y Genética de Interacciones Biológicas (LG2IB), Instituto de Nutrición y Tecnología de los Alimento, Universidad de Chile, El Líbano, Macul, 5524, Santiago, Chile;Center for Research and Innovation in Aquaculture (CRIA), Universidad de Chile, Santiago, Santiago, Chile;GEMA Center for Genomics, Ecology and Environment, Universidad Mayor, Camino La Pirámide, 5750, Huechuraba, Santiago, Chile;Laboratorio de Genómica y Genética de Interacciones Biológicas (LG2IB), Instituto de Nutrición y Tecnología de los Alimento, Universidad de Chile, El Líbano, Macul, 5524, Santiago, Chile;Center for Research and Innovation in Aquaculture (CRIA), Universidad de Chile, Santiago, Santiago, Chile;Laboratorio de Nutrición Animal (LABNA). Facultad de Ciencias Agronómicas, Producción Animal, Universidad de Chile, 11315, Santa Rosa, La Pintana, Chile;

关键词: Piscirickettsia salmonis;    Selenium;    Diet;    Salmonid;    Infection;    Host-directed therapy;   

DOI  :  10.1186/s13567-021-00930-0

来源: Springer

【 摘 要 】

Salmonid Rickettsial Septicaemia (SRS), caused by Piscirickettsia salmonis, is the most important infectious disease in the Chilean salmon farming industry. An opportunity to control this disease is to use functional micronutrients to modulate host mechanisms of response to the infection. Since P. salmonis may affect the host antioxidant system in salmonids, particularly that dependent on selenium (Se), we hypothesized that fish’s dietary selenium supplementation could improve the response to the bacterial infection. To address this, we defined a non-antibiotic, non-cytotoxic concentration of selenium to evaluate its effect on the response to in vitro infections of SHK-1 cells with P. salmonis. The results indicated that selenium supplementation reduced the cytopathic effect, intracellular bacterial load, and cellular mortality of SHK-1 by increasing the abundance and activity of host glutathione peroxidase. We then prepared diets supplemented with selenium up to 1, 5, and 10 mg/kg to feed juvenile trout for 8 weeks. At the end of this feeding period, we obtained their blood plasma and evaluated its ability to protect SHK-1 cells from infection with P. salmonis in ex vivo assays. These results recapitulated the observed ability of selenium to protect against infection with P. salmonis by increasing the concentration of selenium and the antioxidant capacity in fish’s plasma. To the best of our knowledge, this is the first report of the protective capacity of selenium against P. salmonis infection in salmonids, becoming a potential effective host-directed dietary therapy for SRS and other infectious diseases in animals at a non-antibiotic concentration.

【 授权许可】

CC BY