Integrated transcriptome and proteome analysis reveals complex regulatory mechanism of cotton in response to salt stress

Journal of Cotton Research

Haijiang XU¹  Jie KONG¹  Lin CHEN²  Xiyan YANG²  Heng SUN³ 

[1] Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, 830091, Urumqi, Xinjiang, China;National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, Hubei, China;National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, Hubei, China;Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, 430074, Wuhan, Hubei, China;

关键词: Cotton;    Proteome;    Salt stress;    Transcriptome;   

DOI  :  10.1186/s42397-021-00085-5

来源: Springer

【 摘 要 】

BackgroundSoil salt stress seriously restricts the yield and quality of cotton worldwide. To investigate the molecular mechanism of cotton response to salt stress, a main cultivated variety Gossypium hirsutum L. acc. Xinluzhong 54 was used to perform transcriptome and proteome integrated analysis.ResultsThrough transcriptome analysis in cotton leaves under salt stress for 0 h (T0), 3 h (T3) and 12 h (T12), we identified 8 436, 11 628 and 6 311 differentially expressed genes (DEGs) in T3 vs. T0, T12 vs. T0 and T12 vs. T3, respectively. A total of 459 differentially expressed proteins (DEPs) were identified by proteomic analysis, of which 273, 99 and 260 DEPs were identified in T3 vs. T0, T12 vs. T0 and T12 vs. T3, respectively. Metabolic pathways, biosynthesis of secondary metabolites, photosynthesis and plant hormone signal transduction were enriched among the identified DEGs or DEPs. Detail analysis of the DEGs or DEPs revealed that complex signaling pathways, such as abscisic acid (ABA) and jasmonic acid (JA) signaling, calcium signaling, mitogen-activated protein kinase (MAPK) signaling cascade, transcription factors, activation of antioxidant and ion transporters, were participated in regulating salt response in cotton.ConclusionsOur research not only contributed to understand the mechanism of cotton response to salt stress, but also identified nine candidate genes, which might be useful for molecular breeding to improve salt-tolerance in cotton.

【 授权许可】

CC BY