Multi-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600

Biotechnology for Biofuels

Gabriela Felix Persinoti¹  Jennifer Andrea Tamayo Peña²  Michelle Fernandes Araújo²  Cesar Rafael Fanchini Terrasan³  Marcelo Ventura Rubio³  Jaqueline Aline Gerhardt³  Fernanda Lopes de Figueiredo³  Ana Carolina Piva de Oliveira⁴  Thiago Augusto Gonçalves⁵  André Damasio⁶  Geizecler Tomazetto⁷  Sarita Cândida Rabelo⁸  Maria Augusta de Carvalho Silvello⁹  Rosana Goldbeck⁹  Fabio Marcio Squina^1,10  Telma Teixeira Franco^1,11 

[1] Brazilian Biorenewables National Laboratory (LNBr), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, SP, Brazil;Chemical Engineering School, University of Campinas (UNICAMP), Campinas, SP, Brazil;Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil;Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil;Brazilian Biorenewables National Laboratory (LNBr), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, SP, Brazil;Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil;Department of Technological and Environmental Processes, University of Sorocaba (UNISO), Sorocaba, SP, Brazil;Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil;São Paulo Fungal Group, São Paulo, Brazil;Department of Biological and Chemical Engineering (BCE), Aarhus University, 8200, Aarhus, Denmark;Department of Bioprocess and Biotechnology, College of Agricultural Sciences, São Paulo State University (UNESP), Botucatu, SP, Brazil;Department of Food Engineering, Faculty of Food Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil;Department of Technological and Environmental Processes, University of Sorocaba (UNISO), Sorocaba, SP, Brazil;Interdisciplinary Center of Energy Planning (NIPE), University of Campinas (UNICAMP), Campinas, SP, Brazil;

关键词: Basidiomycetes;    Brown-rot;    Genome;    Transcriptome;    Proteome;    CAZymes;    Fenton reaction;    Sugarcane by-products;   

DOI  :  10.1186/s13068-021-01945-7

来源: Springer

【 摘 要 】

BackgroundWood-decay basidiomycetes are effective for the degradation of highly lignified and recalcitrant plant substrates. The degradation of lignocellulosic materials by brown-rot strains is carried out by carbohydrate-active enzymes and non-enzymatic Fenton mechanism. Differences in the lignocellulose catabolism among closely related brown rots are not completely understood. Here, a multi-omics approach provided a global understanding of the strategies employed by L. sulphureus ATCC 52600 for lignocellulose degradation.ResultsThe genome of Laetiporus sulphureus ATCC 52600 was sequenced and phylogenomic analysis supported monophyletic clades for the Order Polyporales and classification of this species within the family Laetiporaceae. Additionally, the plasticity of its metabolism was revealed in growth analysis on mono- and disaccharides, and polysaccharides such as cellulose, hemicelluloses, and polygalacturonic acid. The response of this fungus to the presence of lignocellulosic substrates was analyzed by transcriptomics and proteomics and evidenced the occurrence of an integrated oxidative–hydrolytic metabolism. The transcriptomic profile in response to a short cultivation period on sugarcane bagasse revealed 125 upregulated transcripts, which included CAZymes (redox enzymes and hemicellulases) as well as non-CAZy redox enzymes and genes related to the synthesis of low-molecular-weight compounds. The exoproteome produced in response to extended cultivation time on Avicel, and steam-exploded sugarcane bagasse, sugarcane straw, and Eucalyptus revealed 112 proteins. Contrasting with the mainly oxidative profile observed in the transcriptome, the secretomes showed a diverse hydrolytic repertoire including constitutive cellulases and hemicellulases, in addition to 19 upregulated CAZymes. The secretome induced for 7 days on sugarcane bagasse, representative of the late response, was applied in the saccharification of hydrothermally pretreated grass (sugarcane straw) and softwood (pine) by supplementing a commercial cocktail.ConclusionThis study shows the singularity of L. sulphureus ATCC 52600 compared to other Polyporales brown rots, regarding the presence of cellobiohydrolase and peroxidase class II. The multi-omics analysis reinforces the oxidative–hydrolytic metabolism involved in lignocellulose deconstruction, providing insights into the overall mechanisms as well as specific proteins of each step.

【 授权许可】

CC BY