Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis

Parasites & Vectors

Ana R. Díaz-Marrero¹  José J. Fernández²  Olfa Chiboub³  José E. Piñero⁴  Ines Sifaoui⁴  Jacob Lorenzo-Morales⁴  Manef Abderrabba⁵  Mondher Mejri⁵ 

[1] Instituto Universitario de Bio-Orgánica Antonio González, Universidad de La Laguna (ULL), Avda. Astrofísico Fco. Sánchez 2, 38206, La Laguna, Tenerife, Spain;Instituto Universitario de Bio-Orgánica Antonio González, Universidad de La Laguna (ULL), Avda. Astrofísico Fco. Sánchez 2, 38206, La Laguna, Tenerife, Spain;Departamento de Química Orgánica, Universidad de La Laguna (ULL), Avda. Astrofísico Fco. Sánchez S/N, 38203, La Laguna, Tenerife, Spain;Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Avda. Astrofísico Fco. Sánchez S/N, 38203, La Laguna, Tenerife, Spain;Departamento de Obstetricia y Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna (ULL), La Laguna, Tenerife, Spain;Instituto Universitario de Bio-Orgánica Antonio González, Universidad de La Laguna (ULL), Avda. Astrofísico Fco. Sánchez 2, 38206, La Laguna, Tenerife, Spain;Laboratoire Matériaux-Molécules et Applications, University of Carthage, La Marsa, Carthage, Tunisia;Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Avda. Astrofísico Fco. Sánchez S/N, 38203, La Laguna, Tenerife, Spain;Departamento de Obstetricia y Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna (ULL), La Laguna, Tenerife, Spain;Red de Investigación Cooperativa en Enfermedades Tropicales (RICET), Madrid, Spain;Laboratoire Matériaux-Molécules et Applications, University of Carthage, La Marsa, Carthage, Tunisia;

关键词: T. cruzi;    L. amazonensis;    Diterpenes;    Marine compounds;    Antikinetoplastid;    Apoptosis-like;   

DOI  :  10.1186/s13071-021-04693-7

来源: Springer

【 摘 要 】

BackgroundThe in vitro activity of the brown seaweed Dictyota spiralis against both Leishmania amazonensis and Trypanosoma cruzi was evaluated in a previous study. Processing by bio-guided fractionation resulted in the isolation of three active compounds, classified as diterpenes. In the present study, we performed several assays to detect clinical features associated to cell death in L. amazonensis and T. cruzi with the aim to elucidate the mechanism of action of these compounds on parasitic cells.MethodsThe aims of the experiments were to detect and evaluate specific events involved in apoptosis-like cell death in the kinetoplastid, including DNA condensation, accumulation of reactive oxygen species and changes in ATP concentration, cell permeability and mitochondrial membrane potential, respectively, in treated cells.ResultsThe results demonstrated that the three isolated diterpenes could inhibit the tested parasites by inducing an apoptosis-like cell death.ConclusionsThese results encourage further investigation on the isolated compounds as potential drug candidates against both L. amazonensis and T. cruzi.Graphic abstract

【 授权许可】

CC BY