Low and high-dose intradermal infection with Leishmania majorand Leishmania amazonensis in C57BL/6 mice

Memórias do Instituto Oswaldo Cruz

Denise Fonseca Côrtes²  Matheus Batista Heitor Carneiro²  Liliane Martins Santos²  Talita Correia De Oliveira Souza²  Tatiane Uceli Maioli²  Ana Luiza C Duz¹  Maria Letícia Ramos-jorge¹  Luis Carlos Crocco Afonso¹  Claudia Carneiro¹  Leda Quercia Vieira² 

[1] ,Instituto de Ciências Biológicas Departamento de Bioquímica e Imunologia

关键词: Leishmania;    L. amazonensis;    L. major;    infection;    cytokines;   

DOI  :  10.1590/S0074-02762010000600002

来源: SciELO

【 摘 要 】

A model of skin infection with Leishmania amazonensiswith low doses of parasites is compared to infection with high doses of L. amazonensis and low and high doses of Leishmania major. C57BL/6 mice were infected with 10³ or 10(6) parasites in the ear and the outcome of infection was assessed. The appearance of lesions in mice infected with 10³ parasites was delayed compared to mice infected with 10(6) Leishmania and parasites were detectable at the infection site before lesions became apparent. Mice infected with L. amazonensisdisplayed persistent lesions, whereas infection with L. major spontaneously healed in all groups, although lymphocytes persisted at the site of infection after healing. Macrophages persisted only in L. amazonensis-infected mice. High-dose L. amazonensis-infected mice produced lower levels of IFN-γ and TNF than mice infected with L. major. No correlation between the persistence of parasites and IL-10 levels and the production of nitric oxide or urea by macrophages was found. We conclude that infection with low doses of L. amazonensisin the dermis changes the course of infection by delaying the appearance of lesions. However, low-dose infection does not change the outcomes of susceptibility and cytokine production described for subcutaneous infection with high numbers of parasites.

【 授权许可】

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