Parallel analysis of global garlic gene expression and alliin content following leaf wounding

BMC Plant Biology

G. J. Loake¹  Jihong Jiang²  Junjuan Wang²  Yiren Su²  Xuqin Yang²  Wen Wan²  Xiaoying Cao²  Zhong Zhang²  Jiaying Wu²  Youzhi Wang³  Deliang Ma³  Huijian Chen³ 

[1] Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, EH9 3JH, Edinburgh, UK;The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, 221116, Xuzhou, Jiangsu, China;XuZhou Nuote Chemical co., Ltd., 221137, Xuzhou, Jiangsu, China;

关键词: Garlic;    Allium sativum;    Transcriptomics;    Alliin;    Gene prediction;   

DOI  :  10.1186/s12870-021-02948-0

来源: Springer

【 摘 要 】

BackgroundAllium sativum (garlic) is an economically important food source and medicinal plant rich in sulfides and other protective substances such as alliin, the precursor of allicin biosynthesis. Cysteine, serine and sulfur is the precursor of alliin biosynthesis. However, little is known about the alliin content under abiotic stress or the mechanism by which it is synthesized.ResultsThe findings revealed that the content of alliin was lowest in the garlic roots, and highest in the buds. Furthermore, alliin levels decreased in mature leaves following wounding. Transcriptome data generated over time after wounding further revealed significant up-regulation of genes integral to the biosynthetic pathways of cysteine and serine in mature garlic leaves.ConclusionsThe findings suggest that differential expression of cysteine, serine and sulfide-related genes underlies the accumulation of alliin and its precursors in garlic, providing a basis for further analyses of alliin biosynthesis.

【 授权许可】

CC BY