Microbial community characterization of shrimp survivors to AHPND challenge test treated with an effective shrimp probiotic (Vibrio diabolicus)

Microbiome

Alejandro Reyes¹  Leda Restrepo²  Leandro Bajaña³  Irma Betancourt³  Cristóbal Domínguez-Borbor³  Jenny Rodríguez⁴  Bonny Bayot⁵ 

[1] Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia;Max Planck Tandem Group in Computational Biology, Universidad de los Andes, Bogotá, Colombia;Center for Genome Sciences and Systems Biology, Department of Pathology and Immunology, Washington University in Saint Louis, Saint Louis, MO, USA;Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia;Max Planck Tandem Group in Computational Biology, Universidad de los Andes, Bogotá, Colombia;Escuela Superior Politécnica del Litoral, ESPOL, Centro Nacional de Acuicultura e Investigaciones Marinas, CENAIM, P.O. Box 09-01-5863, Campus Gustavo Galindo Km 30.5 Vía Perimetral, Guayaquil, Ecuador;Escuela Superior Politécnica del Litoral, ESPOL, Centro Nacional de Acuicultura e Investigaciones Marinas, CENAIM, P.O. Box 09-01-5863, Campus Gustavo Galindo Km 30.5 Vía Perimetral, Guayaquil, Ecuador;Escuela Superior Politécnica del Litoral, ESPOL, Centro Nacional de Acuicultura e Investigaciones Marinas, CENAIM, P.O. Box 09-01-5863, Campus Gustavo Galindo Km 30.5 Vía Perimetral, Guayaquil, Ecuador;Escuela Superior Politécnica del Litoral, ESPOL, Facultad de Ciencias de la Vida, FCV, P.O. Box 09-01-5863, Campus Gustavo Galindo Km 30.5 Vía Perimetral, Guayaquil, Ecuador;Escuela Superior Politécnica del Litoral, ESPOL, Centro Nacional de Acuicultura e Investigaciones Marinas, CENAIM, P.O. Box 09-01-5863, Campus Gustavo Galindo Km 30.5 Vía Perimetral, Guayaquil, Ecuador;Escuela Superior Politécnica del Litoral, ESPOL, Facultad de Ingeniería Marítima y Ciencias del Mar, FIMCM, P.O. Box 09-01-5863, Campus Gustavo Galindo Km 30.5 Vía Perimetral, Guayaquil, Ecuador;

关键词: Acute hepatopancreatic necrosis disease (AHPND);    Vibrio diabolicus;    Probiotics;    Metagenomics;    Comparative genomics;    Microbiome;    Horizontal gene transfer (HGT);   

DOI  :  10.1186/s40168-021-01043-8

来源: Springer

【 摘 要 】

BackgroundAcute hepatopancreatic necrosis disease (AHPND) is an important shrimp bacterial disease caused by some Vibrio species. The severity of the impact of this disease on aquaculture worldwide has made it necessary to develop alternatives to prophylactic antibiotics use, such as the application of probiotics. To assess the potential to use probiotics in order to limit the detrimental effects of AHNPD, we evaluated the effect of the ILI strain, a Vibrio sp. bacterium and efficient shrimp probiotic, using metabarcoding (16S rRNA gene) on the gastrointestinal microbiota of shrimp after being challenged with AHPND-causing V. parahaemolyticus.ResultsWe showed how the gastrointestinal microbiome of shrimp varied between healthy and infected organisms. Nevertheless, a challenge of working with AHPND-causing Vibrio pathogens and Vibrio-related bacteria as probiotics is the potential risk of the probiotic strain becoming pathogenic. Consequently, we evaluated whether ILI strain can acquire the plasmid pV-AHPND via horizontal transfer and further cause the disease in shrimp. Conjugation assays were performed resulting in a high frequency (70%) of colonies harboring the pv-AHPND. However, no shrimp mortality was observed when transconjugant colonies of the ILI strain were used in a challenge test using healthy shrimp. We sequenced the genome of the ILI strain and performed comparative genomics analyses using AHPND and non-AHPND Vibrio isolates. Using available phylogenetic and phylogenomics analyses, we reclassified the ILI strain as Vibrio diabolicus. In summary, this work represents an effort to study the role that probiotics play in the normal gastrointestinal shrimp microbiome and in AHPND-infected shrimp, showing that the ILI probiotic was able to control pathogenic bacterial populations in the host's gastrointestinal tract and stimulate the shrimp’s survival. The identification of probiotic bacterial species that are effective in the host’s colonization is important to promote animal health and prevent disease.ConclusionsThis study describes probiotic bacteria capable of controlling pathogenic populations of bacteria in the shrimp gastrointestinal tract. Our work provides new insights into the complex dynamics between shrimp and the changes in the microbiota. It also addresses the practical application of probiotics to solve problems with pathogens that cause high mortality-rate in shrimp farming around the world.5HokUSghi4XQ9JWVrk_iScVideo Abstract

【 授权许可】

CC BY