Portulaca oleracea seeds’ extract alleviates acrylamide-induced testicular dysfunction by promoting oxidative status and steroidogenic pathway in rats

BMC Complementary Medicine and Therapies

Hanan A. Ogaly¹  Reham M. Abd-Elsalam²  Kawkab A. Ahmed²  Muhammad A. Alsherbiny³  Shymaa A. El Badawy⁴  Ola M. Farag⁵ 

[1] Department of Biochemistry, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt;Department of Pathology, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt;Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, 12613, Cairo, Egypt;Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt;General Organization for Veterinary Services, Giza, Egypt;

关键词: Acrylamide;    Oxidative stress;    Histopathology;    Apoptosis;    Portulaca oleracea;    Testosterone;    Steroidogenesis;   

DOI  :  10.1186/s12906-021-03286-2

来源: Springer

【 摘 要 】

BackgroundAcrylamide (ACR) is a widespread industrial and food contaminant that garnered considerable attention for its carcinogenic, neurotoxic, and reproductive toxic effects. The antioxidant effects of Portulaca oleracea seeds extract (POS) and its fertility-enhancing effects were inspiring to evaluate the protective potential and pinpoint the mechanisms and molecular targets of the UPLC-MS fingerprinted POS extract on ACR-induced testicular toxicity in rats.MethodsMale Wistar rats were divided into 6 equal groups of negative control, ACR model (10 mg/kg b.wt.), POS at doses of (200 and 400 mg/kg b.wt.) and POS-treated ACR groups. All treatments were given by oral dosing every day for 60 days.ResultsAdministration of POS extract reversed the ACR-induced epididymides weight loss with improved semen quality and count, ameliorated the ACR-decreased testicular lesion scoring, testicular oxidative stress, testicular degeneration, Leydig cell apoptosis and the dysregulated PCNA and Caspase-3 expression in a dose-dependent manner. It upregulated the declined level of serum testosterone and the expression of steroidogenic genes such as CYP11A1 and 17β3-HSD with an obvious histologic improvement of the testes with re-establishment of the normal spermatogenic series, Sertoli and Leydig cells.ConclusionsThe supplementation with POS extract may provide a potential protective effect for ACR-induced testicular dysfunction which is mediated by its antioxidant, antiapoptotic and steroidogenic modulatory effects.

【 授权许可】

CC BY