Comprehensive circRNA-microRNA-mRNA network analysis revealed the novel regulatory mechanism of Trichosporon asahii infection

Military Medical Research

Ming-Wang Zhang¹  Jun-Hong Ao²  Wan-Ting Luo³  Rong-Ya Yang³  Zhi-Kuan Xia³  Xin Yang³  Zhi-Hong Zhu⁴ 

[1] Department of Dermatology, Southwest Hospital, Army Medical University, 400038, Chongqing, China;Department of Dermatology, The Seventh Medical Center of PLA General Hospital, 5 Nanmencang, Dongcheng District, 100700, Beijing, China;Department of Dermatology, The Seventh Medical Center of PLA General Hospital, 5 Nanmencang, Dongcheng District, 100700, Beijing, China;Department of Dermatology, The Seventh Medical Center of PLA General Hospital, 5 Nanmencang, Dongcheng District, 100700, Beijing, China;The Second School of Clinical Medicine, Southern Medical University, 510280, Guangzhou, Guangdong, China;Department of Ophthalmology, Hainan Hospital of Chinese PLA General Hospital, 572000, Sanya, Hainan, China;

关键词: Trichosporon asahii;    Circular RNA;    Competing endogenous RNA;    RNA sequencing;   

DOI  :  10.1186/s40779-021-00311-w

来源: Springer

【 摘 要 】

BackgroundInvasive Trichosporon asahii (T. asahii) infection frequently occurs with a high mortality in immunodeficient hosts, but the pathogenesis of T. asahii infection remains elusive. Circular RNAs (circRNAs) are a type of endogenous noncoding RNA that participate in various disease processes. However, the mechanism of circRNAs in T. asahii infection remains completely unknown.MethodsRNA sequencing (RNA-seq) was performed to analyze the expression profiles of circRNAs, microRNAs (miRNAs), and mRNAs in THP-1 cells infected with T. asahii or uninfected samples. Some of the RNA-seq results were verified by RT-qPCR. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to analyze the differentially expressed mRNAs. A circRNA-miRNA-mRNA network was constructed and verified by dual-luciferase reporter assay and overexpression experiments.ResultsA total of 46 circRNAs, 412 mRNAs and 47 miRNAs were differentially expressed at 12 h after T. asahii infection. GO and KEGG analyses showed that the differentially expressed mRNAs were primarily linked to the leukocyte migration involved in the inflammatory response, the Toll-like receptor signaling pathway, and the TNF signaling pathway. A competing endogenous RNA (ceRNA) network was constructed with 5 differentially expressed circRNAs, 5 differentially expressed miRNAs and 42 differentially expressed mRNAs. Among them, hsa_circ_0065336 was found to indirectly regulate PTPN11 expression by sponging miR-505-3p.ConclusionsThese data revealed a comprehensive circRNA-associated ceRNA network during T. asahii infection, thus providing new insights into the pathogenesis of the T. asahii-host interactions.

【 授权许可】

CC BY