Organoids: a promising new in vitro platform in livestock and veterinary research

Veterinary Research

Ole Madsen¹  Martien A. M. Groenen¹  Jerry M. Wells²  Henri Woelders³  Soumya K. Kar³  Marinus F. W. te Pas³  Esther D. Ellen³ 

[1]Animal Breeding and Genomics, Wageningen University & Research, Wageningen, The Netherlands

[2]Host-Microbe Interactomics, Wageningen University & Research, Wageningen, The Netherlands

[3]Wageningen Livestock Research, Wageningen University & Research, Wageningen, The Netherlands

关键词: Animal health;    Animal breeding and genomics;    Animal nutrition;    Host-microbe interaction;    In vitro model;    Organoids;    Stem cell research;    Veterinary research;   

DOI  :  10.1186/s13567-021-00904-2

来源: Springer

【 摘 要 】

Organoids are self-organizing, self-renewing three-dimensional cellular structures that resemble organs in structure and function. They can be derived from adult stem cells, embryonic stem cells, or induced pluripotent stem cells. They contain most of the relevant cell types with a topology and cell-to-cell interactions resembling that of the in vivo tissue. The widespread and increasing adoption of organoid-based technologies in human biomedical research is testament to their enormous potential in basic, translational- and applied-research. In a similar fashion there appear to be ample possibilities for research applications of organoids from livestock and companion animals. Furthermore, organoids as in vitro models offer a great possibility to reduce the use of experimental animals. Here, we provide an overview of studies on organoids in livestock and companion animal species, with focus on the methods developed for organoids from a variety of tissues/organs from various animal species and on the applications in veterinary research. Current limitations, and ongoing research to address these limitations, are discussed. Further, we elaborate on a number of fields of research in animal nutrition, host-microbe interactions, animal breeding and genomics, and animal biotechnology, in which organoids may have great potential as an in vitro research tool.

【 授权许可】

CC BY