Pre-adolescence DNA methylation is associated with BMI status change from pre- to post-adolescence

Clinical Epigenetics

John W. Holloway¹  Hongmei Zhang²  Jiajing Wang²  Caroline Relton³  Faisal I. Rezwan⁴  S. Hasan Arshad⁵ 

[1] Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, SO16 6YD, Southampton, UK;Faculty of Medicine, Human Development and Health, University of Southampton, SO16 6YD, Southampton, UK;Division of Epidemiology, Biostatistics, and Environmental Health, School of Public Health, University of Memphis, Memphis, TN, USA;MRC Integrative Epidemiology Unit, University of Bristol, Bristol, UK;Bristol Medical School, Population Health Sciences, University of Bristol, Bristol, UK;School of Water, Energy and Environment, Cranfield University, MK43 0AL, Cranfield, Bedfordshire, UK;The David Hide Asthma and Allergy Research Centre, St Mary’s, Hospital, Parkhurst Road, PO30 5TG, Newport, Isle of Wight, UK;Faculty of Medicine, Human Development and Health, University of Southampton, SO16 6YD, Southampton, UK;

关键词: Adolescence;    DNA methylation;    Longitudinal;    Body mass index (BMI);    BMI status transition;    Obesity;   

DOI  :  10.1186/s13148-021-01042-4

来源: Springer

【 摘 要 】

BackgroundPrevious studies have shown that DNA methylation (DNAm) is associated with body mass index (BMI). However, it is unknown whether DNAm at pre-adolescence is associated with BMI status transition from pre- to post-adolescence. In the Isle of Wight (IoW) birth cohort, genome-wide DNA methylation in whole blood was measured using Illumina Infinium Human450 and EPIC BeadChip arrays in n = 325 subjects, and pre- to post-adolescence BMI transition was classified into four groups: (1) normal to normal, (2) normal to overweight or obese, (3) overweight or obese to normal, and (4) persistent overweight or obese. We used recursive random forest to screen genome-wide Cytosine-phosphate-Guanine (CpG) sites with DNAm potentially associated with BMI transition for each gender, and the association of BMI status transition with DNAm at an earlier age was assessed via logistic regressions. To evaluate gender specificity, interactions between DNAm and gender were included in the model. Findings in the IoW cohort were further tested in an independent cohort, the Avon Longitudinal Study of Parents and Children(ALSPAC).ResultsIn total, 174 candidate CpGs were selected including CpGs from screening and CpGs previously associated correctionally with BMI in children and adults. Of these 174 CpGs, pre-adolescent DNAm of 38 CpGs in the IoW cohort was associated with BMI status transition, including 30 CpGs showing gender-specific associations. Thirteen CpGs showed consistent associations between the IoW cohort and the ALSPAC cohort (11 of which were gender-specific).ConclusionPre-adolescence DNAm is associated with the change in BMI status from pre- to post-adolescence and such associations are likely to be gender-specific.

【 授权许可】

CC BY