Identification of the molecular regulation of differences in lipid deposition in dedifferentiated preadipocytes from different chicken tissues

BMC Genomics

Na Luo¹  Huanxian Cui¹  Lu Liu¹  Jing Li²  Huimin Kang²  Zheng Ma²  Hai Xiang²  Hua Li²  Guiping Zhao³ 

[1]Institute of Animal Sciences, Chinese Academy of Agricultural Sciences

[2] State Key Laboratory of Animal Nutrition, 100193, Beijing, China

[3]School of Life Science and Engineering, Foshan University

[4] Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, 534861, Foshan, China

[5]School of Life Science and Engineering, Foshan University

[6] Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, 534861, Foshan, China

[7]Institute of Animal Sciences, Chinese Academy of Agricultural Sciences

[8] State Key Laboratory of Animal Nutrition, 100193, Beijing, China

关键词: Dedifferentiated preadipocytes;    Different tissue derivation;    Lipid deposition;    DEGs;    Chicken;   

DOI  :  10.1186/s12864-021-07459-8

来源: Springer

【 摘 要 】

BackgroundA body distribution with high intramuscular fat and low abdominal fat is the ideal goal for broiler breeding. Preadipocytes with different origins have differences in terms of metabolism and gene expression. The transcriptome analysis performed in this study of intramuscular preadipocytes (DIMFPs) and adipose tissue-derived preadipocytes (DAFPs) aimed to explore the characteristics of lipid deposition in different chicken preadipocytes by dedifferentiation in vitro.ResultsCompared with DAFPs, the total lipid content in DIMFPs was reduced (P < 0.05). Moreover, 72 DEGs related to lipid metabolism were screened, which were involved in adipocyte differentiation, fatty acid transport and fatty acid synthesis, lipid stabilization, and lipolysis. Among the 72 DEGs, 19 DEGs were enriched in the PPAR signaling pathway, indicating its main contribution to the regulation of the difference in lipid deposition between DAFPs and DIMFPs. Among these 19 genes, the representative APOA1, ADIPOQ, FABP3, FABP4, FABP7, HMGCS2, LPL and RXRG genes were downregulated, but the ACSL1, FABP5, PCK2, PDPK1, PPARG, SCD, SCD5, and SLC27A6 genes were upregulated (P < 0.05 or P < 0.01) in the DIMFPs. In addition, the well-known pathways affecting lipid metabolism (MAPK, TGF-beta and calcium) and the pathways related to cell communication were enriched, which may also contribute to the regulation of lipid deposition. Finally, the regulatory network for the difference in lipid deposition between chicken DAFPs and DIMFPs was proposed based on the above information.ConclusionsOur data suggested a difference in lipid deposition between DIMFPs and DAFPs of chickens in vitro and proposed a molecular regulatory network for the difference in lipid deposition between chicken DAFPs and DIMFPs. The lipid content was significantly increased in DAFPs by the direct mediation of PPAR signaling pathways. These findings provide new insights into the regulation of tissue-specific fat deposition and the optimization of body fat distribution in broilers.

【 授权许可】

CC BY