Accurate determination of genotypic variance of cell wall characteristics of a Populus trichocarpa pedigree using high-throughput pyrolysis-molecular beam mass spectrometry

Biotechnology for Biofuels

Mark F. Davis¹  Gerald A. Tuskan²  David Macaya-Sanz³  Chanaka Roshan Abeyratne³  Stephen P. DiFazio³  Kathleen Haiby⁴  Brian Stanton⁴  Crissa Doeppke⁵  Anne E. Harman-Ware⁵ 

[1] Biosciences Center, National Renewable Energy Laboratory, 80401, Golden, CO, USA;Biosciences Division, Oak Ridge National Laboratory, 37830, Oak Ridge, TN, USA;Department of Biology, West Virginia University, 26506, Morgantown, WV, USA;Greenwood Resources, 97201, Portland, OR, USA;Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, 80401, Golden, CO, USA;

关键词: Biomass composition;    Poplar;    Heritability;    Pyrolysis-molecular beam mass spectrometry;    Phenotypic plasticity;   

DOI  :  10.1186/s13068-021-01908-y

来源: Springer

【 摘 要 】

BackgroundPyrolysis-molecular beam mass spectrometry (py-MBMS) analysis of a pedigree of Populus trichocarpa was performed to study the phenotypic plasticity and heritability of lignin content and lignin monomer composition. Instrumental and microspatial environmental variability were observed in the spectral features and corrected to reveal underlying genetic variance of biomass composition.ResultsLignin-derived ions (including m/z 124, 154, 168, 194, 210 and others) were highly impacted by microspatial environmental variation which demonstrates phenotypic plasticity of lignin composition in Populus trichocarpa biomass. Broad-sense heritability of lignin composition after correcting for microspatial and instrumental variation was determined to be H2 = 0.56 based on py-MBMS ions known to derive from lignin. Heritability of lignin monomeric syringyl/guaiacyl ratio (S/G) was H2 = 0.81. Broad-sense heritability was also high (up to H2 = 0.79) for ions derived from other components of the biomass including phenolics (e.g., salicylates) and C5 sugars (e.g., xylose). Lignin and phenolic ion abundances were primarily driven by maternal effects, and paternal effects were either similar or stronger for the most heritable carbohydrate-derived ions.ConclusionsWe have shown that many biopolymer-derived ions from py-MBMS show substantial phenotypic plasticity in response to microenvironmental variation in plantations. Nevertheless, broad-sense heritability for biomass composition can be quite high after correcting for spatial environmental variation. This work outlines the importance in accounting for instrumental and microspatial environmental variation in biomass composition data for applications in heritability measurements and genomic selection for breeding poplar for renewable fuels and materials.

【 授权许可】

CC BY