Comparative transcriptome analysis reveals ectopic delta-5 and delta-6 desaturases enhance protective gene expression upon Vibrio vulnificus challenge in Tilapia (Oreochromis niloticus)

BMC Genomics

Venugopal Rajanbabu¹  Jen-Leih Wu²  Shin-Jie Huang²  Pin-Yang Tu³  Jyh-Yih Chen⁴ 

[1] Department of Plant Breeding 7 Genetics, Anbil Dharmalingam Agricultural College & Research Institute, Tamil Nadu Agricultural University, 620027, Tiruchirapalli, Tamil Nadu, India;Institute of Cellular and Organismic Biology, Academia Sinica, 128 Academia Road, Section 2, Nankang, 115, Taipei, Taiwan;Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Rd., Jiaushi, 262, Ilan, Taiwan;Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Rd., Jiaushi, 262, Ilan, Taiwan;The iEGG and Animal Biotechnology Center, National Chung Hsing University, 402, Taichung, Taiwan;

关键词: Delta-6 desaturase and delta-5 desaturase (D56);    D56 transgenic tilapia fish;    RNA-seq;    Fatty acid-associated genes;    Immune responsive genes;    Inflammatory genes;   

DOI  :  10.1186/s12864-021-07521-5

来源: Springer

【 摘 要 】

BackgroundTilapia (Oreochromis niloticus) cultures are frequently infected by Vibrio vulnificus, causing major economic losses to production units. Previously, tilapia expressing recombinant delta-5 desaturase and delta-6 desaturase (D56) were found to be resistant to V. vulnificus infection. In this report, we profile the D56-mediated molecular changes underlying this resistance in tilapia. A comparative transcriptome analysis was performed on V. vulnificus-infected wild-type and D56-transgenic tilapia using Illumina’s sequencing-by-synthesis approach. Gene enrichment analysis on differentially expressed unigenes was performed, and the expression patterns were validated by real-time PCR.ResultsComparative transcriptome analysis was performed on RNA-sequence profiles obtained from wild-type and D56-transgenic tilapia at 0, 6 and 24 h post-infection with V. vulnificaus. GO and KEGG gene enrichment analyses showed that D56 regulates several pathways and genes, including fatty acid (FA) metabolism associated, and inflammatory and immune response. Expression of selected FA metabolism-associated, inflammatory and immune responsive genes was validated by qPCR. The inflammatory and immune responsive genes that are modulated by FA-associated D56 likely contribute to the enhanced resistance against V. vulnificus infection in Tilapia.ConclusionsTranscriptome profiling and filtering for two-fold change variation showed that 3795 genes were upregulated and 1839 genes were downregulated in D56-transgenic tilapia. These genes were grouped into pathways, such as FA metabolism, FA elongation, FA biosynthesis, biosynthesis of unsaturated FA, FA degradation, inflammation, immune response, and chemokines. FA-associated genes and immune-related genes were modulated by D56 at 6 h and 24 h post infection with V. vulnificus. The expression patterns of FA-related genes, inflammatory genes, antimicrobial peptide genes and immune responsive genes at 0, 3, 6, 12, 24 and 48 h post-infection suggests these genes are involved in the enhanced resistance of D56 transgenic tilapia to V. vulnificus.

【 授权许可】

CC BY