The impact of cesarean delivery on infant DNA methylation

BMC Pregnancy and Childbirth

Yanjun Zhao¹  Yanhong Ming²  Lisu Huang³  Xia Wang³  Yongjie Liu⁴  Yuexin Gan⁴  Jun Zhang⁴  Qian Chen⁴ 

[1] Department of Child Health Care, Shanghai Children’s Hospital, Shanghai Jiao Tong University, 200040, Shanghai, China;Department of Obstetrics and Gynecology, Hospital of Obstetrics and Gynecology, Fudan University, 200011, Shanghai, China;Department of Pediatrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 200092, Shanghai, China;Ministry of Education-Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, 200092, Shanghai, China;

关键词: Cesarean deliveries on maternal request;    Cord blood;    DNA methylation;    Perinatal epidemiology;   

DOI  :  10.1186/s12884-021-03748-y

来源: Springer

【 摘 要 】

BackgroundMounting evidence suggests that cesarean delivery may have a long-lasting effect on infant health. But the underlying mechanisms remain unclear. This study aims to examine whether cesarean delivery on maternal request without any medical indications (CDMR) impacts DNA methylation status in the umbilical cord blood of the infant.MethodsA cross-sectional study was conducted in Shanghai, China. A total of 70 CDMR and 70 vaginal deliveries (VD) were recruited in 2012. The cord blood DNA methylation status was measured in 30 CDMR and 30 VD newborns using Illumina Infinium Human Methylation 450 K BeadChip. To validate the results, the cord blood DNA methylation status was measured in another 40 CDMR and 40 VD newborns using targeted bisulfite sequencing assay. A total of 497 CpG sites from 40 genes were included in the analysis.ResultsA total of 165 differentially methylated positions (DMPs) exhibited differences in DNA methylation by 10% or more between the CDMR and VD groups, many of which were related to the development of the immune system. Based on the targeted bisulfite sequencing assay, 16 genes (16/22, 72.7%) had higher methylation level in the CDMR group than the VD group. Among them, 5 genes were related to the immune system. After considering the estimation of cell type proportions, there was few significant differences in DNA methylation between CDMR and VD groups.ConclusionsThe DMPs identified between CDMR and VD groups might be largely explained by the cell type proportions. Further studies are needed to examine DNA methylation in each cell type separately.

【 授权许可】

CC BY