Antibiotics-induced intestinal dysbacteriosis caused behavioral alternations and neuronal activation in different brain regions in mice

Molecular Brain

Ke Tu¹  Yuefan Yang²  Peng Cao³  Ming-Ming Zhang³  Xin-Tong Qiu³  Xiao-Jun Wu⁴  Pan Wang⁵  Tao Chen⁵  Hui Yang⁶  Hao Zhang⁶ 

[1] Department of Anesthesiology, General Hospital of Tibet Military District, 850007, Lhasa, Tibet, P. R. China;Department of Biomedical Engineering, The Air Force Medical University, Xi`an, China;Department of Human Anatomy, Histology and Embryology & K.K. Leung Brain Research Centre, The Air Force Medical University, No. 169 Changle West Road, 710032, Xi’an, China;Department of Neurosurgery, Fudan University Shanghai Cancer Center, 270 Dongan Road, 200032, Xuhui, Shanghai, China;Institute of Medical Research, Northwestern Polytechnical University, 710072, Xi`an, Shaanxi, P. R. China;Department of Human Anatomy, Histology and Embryology & K.K. Leung Brain Research Centre, The Air Force Medical University, No. 169 Changle West Road, 710032, Xi’an, China;Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi`an, China;

关键词: Intestinal dysbacteriosis;    Behavior;    Antibiotics;    Probiotics;    Mice;   

DOI  :  10.1186/s13041-021-00759-w

来源: Springer

【 摘 要 】

Antibiotics affect gut microbial composition, leading to Gut–Brain-Axis imbalance and neurobehavioral changes. However, the intestinal dysbacteriosis associated behavior changes are not consistently reported. It is not clear whether these changes are transient or permanent. The neuroprotective effect of probiotics against intestinal dysbacteriosis induced alternations needs to be determined either. In the present study, oral antibiotic mixture including Ampicillin, Streptomycin, and Clindamycin was utilized to induce intestinal dysbacteriosis in mice. Antibiotics application triggered mechanical allodynia in von frey test and spontaneous pain in open field test. It also resulted in increased anxiety and depressive-like behaviors and damaged spatial memory performance. After application of probiotics, the mechanical allodynia and spontaneous pain were alleviated significantly. The anxiety behaviors, depressive-like behaviors and recognitive performance were ameliorative aswell. By using Fos protein as a marker, it is found that the sensory, emotion and memory related brain regions were activated in mice with intestinal dysbacteriosis. Our study is not only helpful for enriching our basic knowledge for understanding the changed pain responses and related brain disorders in antibiotics-induced dysbacteriosis mice, but also beneficial for providing a more comprehensive mechanistic explanation for the regulation of antibiotics and probiotics on gut microbiota and relevant alternations in animal neurological behaviors.

【 授权许可】

CC BY