Molecular and computational approaches to map regulatory elements in 3D chromatin structure

Epigenetics & Chromatin

Suhn K. Rhie¹  Beoung Hun Lee¹ 

[1] Department of Biochemistry and Molecular Medicine and the Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, 90089, Los Angeles, CA, USA;

关键词: Epigenomics;    Regulatory elements;    Chromatin interactions;    Databases;    Analysis tools;   

DOI  :  10.1186/s13072-021-00390-y

来源: Springer

【 摘 要 】

Epigenetic marks do not change the sequence of DNA but affect gene expression in a cell-type specific manner by altering the activities of regulatory elements. Development of new molecular biology assays, sequencing technologies, and computational approaches enables us to profile the human epigenome in three-dimensional structure genome-wide. Here we describe various molecular biology techniques and bioinformatic tools that have been developed to measure the activities of regulatory elements and their chromatin interactions. Moreover, we list currently available three-dimensional epigenomic data sets that are generated in various human cell types and tissues to assist in the design and analysis of research projects.

【 授权许可】

CC BY