Time-dependent efficacy of combination of silver-containing hydroxyapatite coating and vancomycin on methicillin-resistant Staphylococcus aureus biofilm formation in vitro

BMC Research Notes

Sakumo Kii¹  Motoki Sonohata¹  Masaaki Mawatari¹  Akira Hashimoto¹  Tomoki Kobatake¹  Hiroshi Miyamoto²  Takeo Shobuike²  Iwao Noda³ 

[1]Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Nabeshima 5-1-1, 849-8501, Saga, Japan

[2]Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Nabeshima 5-1-1, 849-8501, Saga, Japan

[3]Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Nabeshima 5-1-1, 849-8501, Saga, Japan

[4]Research Section, Medical Division, KYOCERA Corporation, 800 Ichimiyake, 530-2362, Yasu City, Shiga, Japan

关键词: Biofilm;    Hydroxyapatite;    MRSA;    Silver;    Vancomycin;   

DOI  :  10.1186/s13104-021-05499-7

来源: Springer

【 摘 要 】

ObjectiveWe developed a silver-containing hydroxyapatite (Ag-HA) coating to prevent periprosthetic joint infection (PJI). Methicillin-resistant Staphylococcus aureus (MRSA) is the main PJI-causing bacteria. Previously, we had reported the combined effect of Ag-HA coating and vancomycin (VCM) on MRSA biofilm formation 24 h after MRSA inoculation. In this study, we investigated the time-dependent efficacy of Ag-HA coating and VCM on MRSA biofilm formation on Ti discs in vitro by three-dimensional confocal laser scanning microscopic analysis.ResultsFor the Ti VCM and HA VCM groups, the total biofilm volumes per area at 96 h after MRSA inoculation were significantly larger than those at 48 h after MRSA inoculation, respectively (p < 0.001). In contrast, for the Ag-HA VCM group, the total biofilm volume per area at 96 h was significantly smaller than that at 48 h (p < 0.0001). Moreover, 96 h after MRSA inoculation, the total biofilm volume per area of the Ag-HA VCM groups was significantly smaller than those of the Ti VCM and HA VCM groups (p < 0.0001). Thus, the combination of Ag-HA and VCM might be useful for the prevention of MRSA-associated PJI.

【 授权许可】

CC BY