QTLs and candidate genes analyses for fruit size under domestication and differentiation in melon (Cucumis melo L.) based on high resolution maps

BMC Plant Biology

Jordi Garcia-Mas¹  Benxue Chen²  Yongyang Xu³  Guangwei Zhao³  Yuhua He³  Aiai Zhang⁴  Qiushi Fu⁴  Jing Zheng⁴  Huaisong Wang⁴  Zhicheng Hu⁴  Qun Lian⁵  Changsheng Wang⁶  Chuanqiang Xu⁷ 

[1] Centre for Research in Agricultural Genomics CSIC-IRTA-UAB-UB, Barcelona, Spain;Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Barcelona, Spain;Design Gollege, Zhoukou Normal University, 466000, Zhoukou, China;Henan Key Laboratory of Fruit and Cucurbit Biology, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, 450009, Zhengzhou, China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, China;Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, 518000, Shenzhen, China;National Center for Gene Research, CAS Center for Excellence in Molecular Plant Sciences, 200000, Shanghai, China;Shenyang Agricultural University, College of Horticulture, 110866, Shenyang, China;

关键词: Melon;    Genetic map;    Fruit size;    QTL analysis;   

DOI  :  10.1186/s12870-021-02904-y

来源: Springer

【 摘 要 】

BackgroundMelon is a very important horticultural crop produced worldwide with high phenotypic diversity. Fruit size is among the most important domestication and differentiation traits in melon. The molecular mechanisms of fruit size in melon are largely unknown.ResultsTwo high-density genetic maps were constructed by whole-genome resequencing with two F2 segregating populations (WAP and MAP) derived from two crosses (cultivated agrestis × wild agrestis and cultivated melo × cultivated agrestis). We obtained 1,871,671 and 1,976,589 high quality SNPs that show differences between parents in WAP and MAP. A total of 5138 and 5839 recombination events generated 954 bins in WAP and 1027 bins in MAP with the average size of 321.3 Kb and 301.4 Kb respectively. All bins were mapped onto 12 linkage groups in WAP and MAP. The total lengths of two linkage maps were 904.4 cM (WAP) and 874.5 cM (MAP), covering 86.6% and 87.4% of the melon genome. Two loci for fruit size were identified on chromosome 11 in WAP and chromosome 5 in MAP, respectively. An auxin response factor and a YABBY transcription factor were inferred to be the candidate genes for both loci.ConclusionThe high-resolution genetic maps and QTLs analyses for fruit size described here will provide a better understanding the genetic basis of domestication and differentiation, and provide a valuable tool for map-based cloning and molecular marker assisted breeding.

【 授权许可】

CC BY