Effect of six weeks 1000 mg/day vitamin C supplementation and healthy training in elderly women on genes expression associated with the immune response - a randomized controlled trial

Journal of the International Society of Sports Nutrition

Agata Grzybkowska¹  Jędrzej Antosiewicz²  Mariusz Zasada³  Danuta Dworakowska³  Małgorzata Żychowska⁴  Olga Czerwińska-Ledwig⁵  Anna Piotrowska⁵  Wanda Pilch⁵ 

[1] Department of Biochemistry, Faculty of Physical Education, Gdansk University of Physical Education and Sport, Kazimierza Gorskiego 1, 80-336, Gdansk, Poland;Department of Bioenergetics and Exercise Physiology, Faculty of Health Sciences with Institute of Maritime and Tropical Medicine, Medical University of Gdansk, Debinki 1, 80-211, Gdansk, Poland;Department of Sport, Faculty of Physical Education, Kazimierz Wielki University in Bydgoszcz, Jana Karola Chodkiewicza 30, 85-064, Bydgoszcz, Poland;Department of Sport, Faculty of Physical Education, Kazimierz Wielki University in Bydgoszcz, Jana Karola Chodkiewicza 30, 85-064, Bydgoszcz, Poland;Department of Biochemistry, Faculty of Physical Education, Gdansk University of Physical Education and Sport, Kazimierza Gorskiego 1, 80-336, Gdansk, Poland;Institute for Basic Sciences, Faculty of Physiotherapy, University of Physical Education in Krakow, Jana Pawła II 78, 31-571, Krakow, Poland;

关键词: Supplementation;    Aging;    Genes involved in immune response;   

DOI  :  10.1186/s12970-021-00416-6

来源: Springer

【 摘 要 】

BackgroundIn this study, we investigated the effects of supplementation and exercise on the expression of genes associated with inflammation like CCL2, CRP, IL1, IL6, IL10 mRNA in elderly women.MethodsTwenty four participants divided randomly into two groups were subjected to 6 weeks of the same health training program (three times per week). SUP group (supplemented, n = 12, mean age 72.8 ± 5.26 years and mean body mass 68.1 ± 8.3 kg) received 1000 mg of Vitamin C/day during the training period, while CON group (control, n = 12, mean age 72.4 ± 5.5 years and body mass 67.7 ± 7.5 kg) received placebo.ResultsNo significant changes in IL-1, IL-6, IL-10 and CRP mRNA were observed within and between groups. However, there was a clear tendency of a decrease in IL-6 (two-way ANOVA, significant between investigated time points) and an increase in IL-10 mRNA noted in the supplemented group. A significant decrease in CCL2 mRNA was observed only in the CON group (from 2^0.2 to 2^0.1, p = 0.01).ConclusionsIt can be concluded, that 6 weeks of supplementation and exercise was too short to obtain significant changes in gene expression in leukocytes, but supplementation of 1000 mg vitamin C positively affected IL-6 and IL-10 expression – which are key changes in the adaptation to training. However, changes in body mass, IL1 and CCL2 were positive in CON group. It is possible that Vitamin C during 6 weeks of supplementation could have different effects on the expression of individual genes involved in the immune response.Trial registrationRetrospectively registered. 

【 授权许可】

CC BY