The small GTPase ARF-1.2 is a regulator of unicellular tube formation in Caenorhabditis elegans

The journal of physiological sciences

article

Eriko Kage-Nakadai¹  Simo Sun²  Satoru Iwata¹  Sawako Yoshina¹  Yoshikazu Nishikawa²  Shohei Mitani¹ 

[1] Department of Physiology, Tokyo Women’s Medical University School of Medicine;Graduate School of Human Life Science, Osaka City University

关键词: Caenorhabditis elegans;    Unicellular tube formation;    Small GTPase;    ARF-1.2;    CDC-42;   

DOI  :  10.1007/s12576-018-0617-5

来源: Springer

【 摘 要 】

The membrane trafficking events that regulate unicellular tube formation and maintenance are not well understood. Here, using an RNAi screen, we identified the small GTPase ARF1 homolog ARF-1.2 as a regulator of excretory tube formation in Caenorhabditis elegans. RNAi-mediated knockdown and knockout of the arf-1.2 gene resulted in the formation of large intracellular vacuoles at the growth sites (varicosities) of the excretory canals. arf-1.2 mutant animals were sensitive to hyperosmotic conditions. arf-1.2 RNAi affected the localization of the anion transporter SULP-8, which is expressed in the basal plasma membrane of the excretory canals, but did not affect the expression of SULP-4, which is expressed in the apical membrane. The phenotype of arf-1.2 mutants was suppressed by mutation of the small Rho GTPase CDC-42, a regulator of apical/basal traffic balance. These results suggest that ARF-1.2 plays an essential role in basal membrane traffic to regulate the formation of the unicellular excretory tube.

【 授权许可】

Unknown