Xanthoceraside prevented synaptic loss and reversed learning-memory deficits in APP/PS1 transgenic mice

The journal of physiological sciences

article

Ge Jin¹  Lin Zhu¹  Peng Liu¹  Qian Xu¹  Yue Qi¹  Xiaoyu Zhou¹  Jikai Xu¹  Xuefei Ji¹  Tianyan Chi¹  Libo Zou¹ 

[1] Department of Pharmacology, Life Science and Biopharmaceutics School, Shenyang Pharmaceutical University;Shenyang Medical College

关键词: Xanthoceraside;    Learning and memory;    Synaptic structure;    BDNF;    TrkB;   

DOI  :  10.1007/s12576-019-00664-x

来源: Springer

【 摘 要 】

Xanthoceraside, a novel triterpenoid saponin, has been found to attenuate learning and memory impairments in AD animal models. However, whether xanthoceraside has a positive effect on synaptic morphology remains unclear. Herein, we evaluated the effects of xanthoceraside on learning and memory impairments and the abnormalities of synaptic structure in APP/PS1 transgenic mice. The behavioral experiments demonstrated that xanthoceraside attenuated the imaginal memory and spatial learning impairments, and improved social interaction. Transmission electron microscopy and Golgi staining showed that xanthoceraside ameliorated synapse morphology abnormalities and dendritic spine density deficits, respectively. Western-blot analysis identified that xanthoceraside increased the expression of SYP and PSD95, activated BDNF/TrkB/MAPK/ERK and PI3K/Akt signaling pathways, meanwhile decreased the expression of RhoA, ROCK and Snk, increased the levels of SPAR, and activated the BDNF/TrkB/cofilin signaling pathway. Taken together, our study indicated that xanthoceraside improved cognitive function and protected both synaptic morphology and dendritic spine in APP/PS1 transgenic mice, which might be related in part to its activation in the BDNF/TrkB pathway.

【 授权许可】

Unknown