Cardiac specific transcription factor Csx/Nkx2.5 regulates transient-outward K + channel expression in pluripotent P19 cell-derived cardiomyocytes

The journal of physiological sciences

article

Tomoko Uchino¹  Ming-Qi Zheng¹  Yan Wang¹  Katsushige Ono¹ 

[1] Department of Pathophysiology, Oita University School of Medicine;Department of Anesthesiology, Oita University School of Medicine;Department of Cardiovascular Medicine, Hebei Medical University

关键词: Potassium channel;    Csx/Nkx2.5;    Cardiomyocytes;    Transient outward current;    Cardiogenesis;    Pluripotency;    P19CL6;    Homeobox;   

DOI  :  10.1186/s12576-020-00748-z

来源: Springer

【 摘 要 】

The homeobox-containing gene Csx/Nkx2.5 codes several cardiac transcription factors and plays a critical role in early cardiogenesis. We investigated the effect of Csx/Nkx2.5 on the expression of cardiac ion channels using P19-derived cardiomyocytes. P19CL6 cells and P19CL6 cells with Csx/Nkx2.5 overexpression (P19CL6-Csx cells) were induced to differentiate into cardiomyocytes by treatment with dimethyl sulfoxide. Action potentials and membrane currents were measured by whole cell patch clamp at different differentiation stage: the early stage (1–5 days after beating had begun) and the late stage (10–15 days after beating). Expression of Csx/Nkx2.5 mRNA was increased as the differentiation stages advanced in both P19CL6 and P19CL6-Csx cells. In action potential configuration, maximal diastolic potentials in P19CL6-Csx cells exhibited more hyperpolarized potential (‒ 64.2 mV) than those in P19CL6 cells (‒ 54.8 mV, p < 0.01) in the early stage. In P19CL6 cells, among 6 different voltage-gated and ligand-operated K+ channels expressed during the early stage, the transient-outward K+ channel was most predominant. By overexpression of Csx/Nkx2.5, developmental decrease in the transient-outward K+ channel was suppressed. Homeobox-containing gene Csx/Nkx2.5 modifies the amount of distinct ionic channels, during differentiation periods, predominantly changing the expression of the transient-outward K+ channel.

【 授权许可】

Unknown