Safe range of shortening the middle thoracic spine, an experimental study in canine

European spine journal

article

Le Ji¹  Xiaoying Ma²  Wenchen Ji³  Shengli Huang⁴  Min Feng¹  Jingyuan Li¹  Lisong Heng⁵  Yajuan Huang⁴  Binshang Lan⁴ 

[1] Department of Orthopedic Surgery, The Third Affiliated Hospital of Xi’an Jiaotong University (Shaanxi Provincial People’s Hospital);Department of Gastroenterology, The Third Affiliated Hospital of Xi’an Jiaotong University (Shaanxi Provincial People’s Hospital);Department of Orthopedic Surgery, The First Affiliated Hospital of Xi’an Jiaotong University;Department of Orthopedic Surgery, The Second Affiliated Hospital of Xi’an Jiaotong University;Department of Orthopedic Surgery, Honghui Hospital

关键词: Spinal cord shortening;    Spinal cord injury;    Spinal cord blood fow;    Somatosensory evoked potential;    Bloodspinal cord barrier;    Macrophage;    Inducible nitric oxide synthase;   

DOI  :  10.1007/s00586-019-06268-8

来源: Springer

【 摘 要 】

To determine the safe range of shortening the spinal column at middle thoracic spine and to observe the changes in blood-spinal cord barrier (BSCB), microglia/macrophage activation and inducible nitric oxide synthase (iNOS) activity after shortening-induced spinal cord injury. Dogs were allocated to four groups. Group A (control) underwent laminectomy of T7 without shortening the spinal column. Groups B, C and D had 1/3, 1/2, and 2/3 of T7 resected, respectively, followed by spinal shortening. Somatosensory evoked potential (SSEP) and hind-limb function were recorded periodically for 14 days after operation. Spinal cord blood flow (SCBF) and BSCB were detected at the acute phase of shortening. Microglia/macrophage reactions and iNOS activity were observed by immunohistochemistry. Shortening of 1/3 of a vertebral height caused no significant changes in SSEP and hind-limb function after operation, whereas shortening of 1/2 of the height caused SSEP abnormality and paraparesis, and severe neurologic deficit of hind-limb was observed when the shortening reached 2/3 of the height. SCBF increased temporarily and showed a trend of recovery when the shortening was within 1/2 of a vertebral segment height. When it reached 1/2 or 2/3 of the height, SCBF at 6 h post-operation was 86.33% or 74.95% of the baseline, and an increasing BSCB permeability was observed. In the subsequent 7 days, obvious activation of macrophage and increased number of iNOS-positive cells were observed. It is safe to shorten the spinal cord within 1/3 of a vertebral height in middle thoracic spine under two-segment laminectomy in canine. The BSCB disruption, macrophage activation, and increased iNOS activity were observed in the acute phase of the injury. These slides can be retrieved under Electronic Supplementary Material.

【 授权许可】

Unknown