Integrated metabolic profiling and transcriptome analysis of pigment accumulation in Lonicera japonica flower petals during colour-transition

BMC Plant Biology

Yan Xia¹  Di Wu¹  Shuming Wang¹  Dan Wang¹  Baogui Xue¹  Lehua Xing¹  Guolu Liang¹  Xinya Liu¹  Qigao Guo¹  Weiwei Chen²  Weibo Xiang³ 

[1] Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education; College of Horticulture and Landscape Architecture, Southwest University, 400715, Chongqing, China;Academy of Agricultural Sciences of Southwest University, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, 400715, Chongqing, China;Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education; College of Horticulture and Landscape Architecture, Southwest University, 400715, Chongqing, China;Henan International Joint Laboratory of Crop Gene Resources and Improvement, School of Agricultural Sciences, Zhengzhou University, 450001, Zhengzhou, Henan, China;Rare Plant Research Institute of the Yangtze River (Yichang); Institute of Science and Technology, China Three Gorges Corporation, 100083, Beijing, China;

关键词: Lonicera japonica;    Petal colour;    Pigment;    Gene expression;    Endogenous hormones;   

DOI  :  10.1186/s12870-021-02877-y

来源: Springer

【 摘 要 】

BackgroundPlants have remarkable diversity in petal colour through the biosynthesis and accumulation of various pigments. To better understand the mechanisms regulating petal pigmentation in Lonicera japonica, we used multiple approaches to investigate the changes in carotenoids, anthocyanins, endogenous hormones and gene expression dynamics during petal colour transitions, i.e., green bud petals (GB_Pe), white flower petals (WF_Pe) and yellow flower petals (YF_Pe).ResultsMetabolome analysis showed that YF_Pe contained a much higher content of carotenoids than GB_Pe and WF_Pe, with α-carotene, zeaxanthin, violaxanthin and γ-carotene identified as the major carotenoid compounds in YF_Pe. Comparative transcriptome analysis revealed that the key differentially expressed genes (DEGs) involved in carotenoid biosynthesis, such as phytoene synthase, phytoene desaturase and ζ-carotene desaturase, were significantly upregulated in YF_Pe. The results indicated that upregulated carotenoid concentrations and carotenoid biosynthesis-related genes predominantly promote colour transition. Meanwhile, two anthocyanins (pelargonidin and cyanidin) were significantly increased in YF_Pe, and the expression level of an anthocyanidin synthase gene was significantly upregulated, suggesting that anthocyanins may contribute to vivid yellow colour in YF_Pe. Furthermore, analyses of changes in indoleacetic acid, zeatin riboside, gibberellic acid, brassinosteroid (BR), methyl jasmonate and abscisic acid (ABA) levels indicated that colour transitions are regulated by endogenous hormones. The DEGs involved in the auxin, cytokinin, gibberellin, BR, jasmonic acid and ABA signalling pathways were enriched and associated with petal colour transitions.ConclusionOur results provide global insight into the pigment accumulation and the regulatory mechanisms underlying petal colour transitions during the flower development process in L. japonica.

【 授权许可】

CC BY