Adults with Prader–Willi syndrome exhibit a unique microbiota profile

BMC Research Notes

Wendy J. Dahl¹  Zainab Alyousif¹  Jennifer L. Miller²  Thomas A. Tompkins³  Jérémie Auger³ 

[1] Department of Food Science and Human Nutrition, University of Florida, 359 Newell Drive, 32611, Gainesville, FL, USA;Division of Endocrinology, Department of Pediatrics, College of Medicine, University of Florida, 1600 SW Archer Road, 32610, Gainesville, FL, USA;Rosell Institute for Microbiome and Probiotics, 6100 Royalmount, H4P 2R2, Montreal, QC, Canada;

关键词: Prader–Willi syndrome;    RF39;    Blautia;    Tenericutes;    Microbiota;    16S rRNA;   

DOI  :  10.1186/s13104-021-05470-6

来源: Springer

【 摘 要 】

ObjectiveAdults with Prader–Willi syndrome (PWS) require less energy intake to maintain body weight than the general adult population. This, combined with their altered gastrointestinal transit time, may impact microbiota composition. The aim of the study was to determine if the fecal microbiota composition of adults with PWS differed from non-affected adults. Using usual diet/non-interventional samples, fecal microbiota composition was analyzed using 16S rRNA gene amplicon sequencing and data from adults with PWS were merged with four other adult cohorts that differed by geographical location and age. QIIME 2™ sample-classifier, machine learning algorithms were used to cross-train the samples and predict from which dataset the taxonomic profiles belong. Taxa that most distinguished between all datasets were extracted and a visual inspection of the R library PiratePlots was performed to select the taxa that differed in abundance specific to PWS.ResultsFecal microbiota composition of adults with PWS showed low Blautia and enhanced RF39 (phyla Tenericutes), Ruminococcaceae, Alistipes, Erysipelotrichacaea, Parabacteriodes and Odoribacter. Higher abundance of Tenericutes, in particular, may be a signature characteristic of the PWS microbiota although its relationship, if any, to metabolic health is not yet known.

【 授权许可】

CC BY