Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in vitro study

BMC Oral Health

Paolo Ghensi¹  Erica Tambone²  Francesca Agostinacchio²  Devid Maniglio²  Emiliana Bonomi³  Francesco Tessarolo⁴  Giandomenico Nollo⁴  Federico Piccoli⁵  Patrizio Caciagli⁵  Iole Caola⁵  Chiara Ceresa⁶  Letizia Fracchia⁶ 

[1] Department CIBIO, University of Trento, 38123, Trento, Italy;Department of Industrial Engineering and BIOtech, University of Trento, via Sommarive, 38123, Trento, Italy;Department of Industrial Engineering and BIOtech, University of Trento, via Sommarive, 38123, Trento, Italy;Department of Laboratory Medicine, Azienda Provinciale Per I Servizi Sanitari, 38122, Trento, Italy;Department of Industrial Engineering and BIOtech, University of Trento, via Sommarive, 38123, Trento, Italy;Healthcare Research and Innovation Program (IRCS-FBK-PAT), Bruno Kessler Foundation, 38123, Trento, Italy;Department of Laboratory Medicine, Azienda Provinciale Per I Servizi Sanitari, 38122, Trento, Italy;Department of Pharmaceutical Sciences, Università del Piemonte Orientale “A. Avogadro”, 28100, Novara, Italy;

关键词: Dental implants;    Biofilm;    Titanium;    Biosurfactant;    Staphylococcus;   

DOI  :  10.1186/s12903-021-01412-7

来源: Springer

【 摘 要 】

BackgroundPeri-implant mucositis and peri-implantitis are biofilm-related diseases causing major concern in oral implantology, requiring complex anti-infective procedures or implant removal. Microbial biosurfactants emerged as new anti-biofilm agents for coating implantable devices preserving biocompatibility. This study aimed to assess the efficacy of rhamnolipid biosurfactant R89 (R89BS) to reduce Staphylococcus aureus and Staphylococcus epidermidis biofilm formation on titanium.MethodsR89BS was physically adsorbed on titanium discs (TDs). Cytotoxicity of coated TDs was evaluated on normal lung fibroblasts (MRC5) using a lactate dehydrogenase assay. The ability of coated TDs to inhibit biofilm formation was evaluated by quantifying biofilm biomass and cell metabolic activity, at different time-points, with respect to uncoated controls. A qualitative analysis of sessile bacteria was also performed by scanning electron microscopy.ResultsR89BS-coated discs showed no cytotoxic effects. TDs coated with 4 mg/mL R89BS inhibited the biofilm biomass of S. aureus by 99%, 47% and 7% and of S. epidermidis by 54%, 29%, and 10% at 24, 48 and 72 h respectively. A significant reduction of the biofilm metabolic activity was also documented. The same coating applied on three commercial implant surfaces resulted in a biomass inhibition higher than 90% for S. aureus, and up to 78% for S. epidermidis at 24 h.ConclusionsR89BS-coating was effective in reducing Staphylococcus biofilm formation at the titanium implant surface. The anti-biofilm action can be obtained on several different commercially available implant surfaces, independently of their surface morphology.

【 授权许可】

CC BY