Detection of Ciprofloxacin resistance genes in Escherichia coli isolated from dogs with urinary tract infections

The Iranian Journal of Veterinary Science and Technology

article

Mustapha, Muhammad¹  Goel, Parveen¹ 

[1] Department of Veterinary Medicine, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences;Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Maiduguri

关键词: Dog;    Uropathogenic E;    coli;    Antibiotic-resistant genes;    Ciprofloxacin;    PCR;   

DOI  :  10.22067/veterinary.v12i1.86316

学科分类：医学（综合）

来源: Ferdowsi University of Mashhad

【 摘 要 】

This research was performed on uropathogenic Escherichia coli (E. coli) isolates and established the genes of resistance to ciprofloxacin between the isolates. A total of one hundred and three urine samples were tested for uropathogenic E. coli which were obtained from dogs with urinary tract infections (UTIs) using cultural isolation, antimicrobial susceptibility test, and polymerase chain reaction (PCR). The results revealed that genes associated with ciprofloxacin resistance are 24.3% positive for E. coli. The E. coli isolates were resistant to both ciprofloxacin and ampicillin (100%), highly susceptible to chloramphenicol (84.0%), and less susceptible to gentamycin (44.0%) and amikacin (40.0%). The PCR tests showed the presence of the ParC (in 25 samples; 100%), GyrA (in 25 samples; 100%), and GyrB (in 4 samples; 16.0%) genes. The findings of the present study showed an upsetting rate of ciprofloxacin and ampicillin resistance among the E. coli isolates from dogs with UTIs.

【 授权许可】

CC BY