Effect of cobalt nano-particles on serum biochemical and histopathological changes in liver and kidney of lambs

The Iranian Journal of Veterinary Science and Technology

article

Ghoreishi, Seyed Morteza¹  Najafzadeh, Hossein²  Mohammadian, Babak³  Rahimi, Eisa⁴  Afzalzadeh, Mohammad Reza¹  Kazemi Varnamkhasti, Mohammad¹  Ganjeali Darani,, Hadi¹ 

[1] Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz;Department of Pharmacology & Toxicology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz;Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz;Department of Chemistry, Payame Noor University

关键词: Cobalt nano-particles;    hepatotoxicity;    renal toxicity;    lamb;   

DOI  :  10.22067/veterinary.v5i2.16589

学科分类：医学（综合）

来源: Ferdowsi University of Mashhad

【 摘 要 】

Cobalt is an essential co-factor in red blood cell production and function and its deficiency may produce clinical signs in sheep. Thus, present study was designed to evaluate the effect of cobalt nano-particles on serum biochemical factors and histopathological changes in liver and kidnies of lambs. Study was carried out in 3 groups of lambs (4 lambs per group). One group of lambs was kept as control group. Second and third group respectively received cobalt nano-particles and conventional cobalt chloride suspension daily for a period 25 day. Blood sample and then serum was collected before and at the end of study. Activity of ALT,AST, ALP and level of BUN, creatinine and vitamin B12 were measured in serum of lambs. Tissue sections of liver and kidney were stained with hematoxylin and eosin and examined by light microscopy. Activity of ALT, BUN and vitamin B12 was significantly increased by cobalt nano-particles and conventional cobalt chloride. Fatty change of hepatocytes occured by conventional cobalt and granulomatous hepatitis, focal necrosis of hepatocytes and degeneration of hepatocytes by the nano cobalt was identified in liver. There were not any significant lesions and alteration in the kidnies of treated groups. Thus, cobalt nano-particles have similar effect to conventional cobalt for using in sheep with cobalt deficiency.

【 授权许可】

CC BY