Evaluation of the Possible Role of miRNAs in Chemical Allergen Potency

Turkish Journal of Pharmaceutical Sciences

article

Hatice Gül ANLAR¹  Valentina GALBIATI²  Emanuela CORSINI²  Nurşen BAŞARAN³ 

[1] Zonguldak Bülent Ecevit University Faculty of Pharmacy, Department of Pharmaceutical Toxicology;Università degli Studi di Milano, Department of Environmental Science and Policy;Hacettepe University Faculty of Pharmacy, Department of Pharmaceutical Toxicology

关键词: MicroRNA;    miRNA;    immune system;    allergic contact dermatitis;    skin;   

DOI  :  10.4274/tjps.galenos.2019.25349

来源: Galenos Yayinevi

【 摘 要 】

Objectives: MicroRNAs (miRNAs) are short, endogenous noncoding RNA molecules that can bind to certain parts of target mRNAs, thereby regulating gene expression. Studies showed that miRNAs could be up- or downregulated in different allergic skin conditions but there is still need for further studies. The aim of this study was to investigate the expression of miRNAs in response to the common contact allergen Bandrowski’s base (BB), the principal allergen in patients reacting to p-phenylenediamine (PPD). Materials and Methods: The human promyelocytic cell line THP-1 was exposed to BB at a concentration of 1 µg/mL for 24, 48, and 72 h. The dose was selected from the results of cytotoxicity assays. RNA was purified and miRNA expression profile and real-time polymerase chain reaction (RTPCR) were performed to identify up- or downregulated miRNAs and confirm their modulations. Results: Among the different modulated miRNAs, the upregulation of miRNA-155 and the downregulation of miRNA-21 were found to be important because these are related to immune system. This expression profile of miRNAs was also confirmed by RT-PCR. Conclusion: These preliminary results showed that miR-155 and miR-21 may play a role in the pathogenesis of allergic contact dermatitis, but further studies are needed to clarify their definite roles.

【 授权许可】

Unknown