Considering the Effect of Rosa damascena Mill. Essential Oil on Oxidative Stress and COX-2 Gene Expression in the Liver of Septic Rats

Turkish Journal of Pharmaceutical Sciences

article

Abolfazl DADKHAH¹  Faezeh FATEMI²  Mohammad Reza Mohammadi MALAYERI³  Mohammad Hassan Karvin ASHTIYANI⁴  Sakineh Kazemi NOUREINI⁴  Azadeh RASOOLI⁵ 

[1] Islamic Azad University, Qom Branch, Faculty of Medicine, Department of Medicine;Nuclear Science and Technology Research Institute, Materials and Nuclear Fuel Research School;Islamic Azad University, Garmsar Branch, Faculty of Veterinary Medicine, Department of Pathobiology;Hakim Sabzevari University, Faculty of Science, Department of Biology;Payame-e-Noor University, Faculty of Sciences, Department of Biochemistry

关键词: Sepsis;    Rosa damascena Mill.;    oxidative stress;    CLP;    hepatoprotective activity;   

DOI  :  10.4274/tjps.galenos.2018.58815

来源: Galenos Yayinevi

【 摘 要 】

Objectives: Sepsis is a clinical illness with a high rate of mortality all over the world. Oxidative stress is considered the main phenomenon that occurs in sepsis. Rosa damascena Mill. is an ancient herbal plant with high pharmacological activities. Materials and Methods: Cecal ligation and puncture (CLP) as a standard model was used to induce sepsis in rats. Male adult rats were randomly divided into 5 groups. Different doses of R. damascena essential oil (50 and 100 mg/kg.bw) were gavaged orally for 14 days and on day 15 CLP was performed. After 24 h, blood samples and liver tissues were removed in order to measure oxidative stress [myeloperoxidase (MPO), malondialdehyde (MDA), glutathione (GSH), glutathione-S-transferase, and ferric reducing ability of plasma (FRAP)] and biochemical parameters [alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT), and bilirubin] together with plasma prostaglandin E2 (PGE2 ) and COX-2 expression. Results: The essential oil was capable of modulating all of the oxidative stress, antioxidant, and anti-inflammatory parameters induced by CLP as characterized by elevations in MPO and MDA levels as well as increases in AST and ALT concentrations concomitant with PGE2 and COX-2 increments. The antioxidant defense system such as GSH and FRAP was also increased in the essential oil treated groups. Conclusion: Our results showed that the essential oil has antioxidative and hepatoprotective activities through reducing the oxidative injury in sepsis caused by CLP.

【 授权许可】

Unknown