In Vitro Macrophage Nitric Oxide and Interleukin-1 Beta Suppression by Moringa peregrina Seed

Turkish Journal of Pharmaceutical Sciences

article

Shaymaa Fadhel Abbas ALBAAYIT¹  Ahmed Salim Kadhim AL-KHAFAJI¹  Hiba Sarmed ALNAIMY¹ 

[1] University of Baghdad, Faculty of Science, Department of Biology, Iraq & University of Malaya, Faculty of Science, Institute of Biological Sciences

关键词: Moringa peregrina;    nitric oxide;    interleukin-1β;    inflammation;   

DOI  :  10.4274/tjps.galenos.2018.52244

来源: Galenos Yayinevi

【 摘 要 】

Objectives: Moringa peregrina has long been used in folk medicine to treat diseases including fever, headache, burns, constipation, gut pains, and inflammation. Nitric oxide (NO) and interleukin-1β (IL-1β) play an important role in the pathophysiology of inflammation. The objectives of this study were to determine the effect of M. peregrina seed ethanolic extract (MPSE) on the viability of and NO and IL-1β production by lipopolysaccharide (LPS)-activated macrophage (J774A.1) cell line. Materials and Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay was used to determine the cytotoxic effect of MPSE treatment at concentrations ranging from 31.15 to 1000 µg/mL. The NO concentration was determined by Griess assay and IL-1β proinflammatory cytokine concentration by enzyme-linked immunosorbent assay in the supernatant of MPSE-treated LPS-activated J774A.1 cell culture. Results: The results show that the MPSE was not cytotoxic at 1000 μg/mL but significantly (p<0.001) inhibited NO and IL-1β production by the LPS-activated macrophage J774A.1 cells. Conclusion: These findings suggest that M. peregrina seed extract can be used to treat and prevent inflammatory diseases through the inhibition of inflammatory mediators.

【 授权许可】

Unknown