Comparative study between two media on in vitro maturation rate of local buffalo oocytes

Al-Qadisiyah Journal of Veterinary Medicine Sciences

ARTICLE

Ihsan A. Habeeb¹  Suhailla O. Hussain² 

[1] College of Veterinary Medicine, University of Basrah;College of Veterinary Medicine, University of Baghdad

关键词: Buffalo;    Cumulus‐oocyte‐complexes;    IVM;    TCM-199;    Synthetic oviduct.;   

DOI  :  10.29079/vol17iss1art466

学科分类：土木及结构工程学

来源: College of Veterinary Medicine

【 摘 要 】

Availability of developmentally competent buffalo oocytes is critical for in vitro embryo production and application of related biotechniques. The present study has been undertaken to study the effect of TCM-199 and synthetic oviduct fluid (SOF) medium with supplements (10% Bovine serum albumen (BSA), 10 IU/ml follicle stimulating hormone FSH) with100 IU/mL penicillin, 100 mg/mL streptomycin and 100 IU/mL Nystatin on maturation of buffalo oocytes in vitro. Buffalo ovaries (n= 300) were collected from local abattoir of Basrah within two hours of slaughter and transported immediately to the laboratory under septic condition. Follicular oocytes (n= 215) were recovered by aspiration and slicing method. Only normal grades(A and B) cumulus-oocyte-complexes (COCs) were incubating under 5 % CO2 in air at 38.5 C for 24 hours in two media TCM199 media (n= 105) or SOF media( n =110) oocytes adding same supplements which mention above. The percentage of oocytes maturation when using TCM-199 medium was (33.33%) was significantly higher (P<0.01) compared to those obtained with SOF medium 13.91(%).

【 授权许可】

CC BY-NC