Electrical Activation Enhances Pre-Implantation Embryo Development Following Sperm Injection into In Vitro Matured Pig Oocytes

Journal of Veterinary Medical Science

Jae-Gyu YOO⁴  Jong-Yi PARK⁵  Kyu-Chan HWANG⁵  Jae-Hwan KIM³  Jin-Hoi KIM⁵  Chang-Gi HUR¹  Mi-Rung PARK⁵  Seong-Keun CHO² 

[1] CHO-A Biotechnology Research Institute, CHO-A Pharmaceutical Co. Ltd., Seoul 150-992, Republic of Korea;Department of Animal Science, Pusan National University, Gyeongnam 627-706, Republic of Korea;Department of Biomedical Science, College of Life Science, CHA University, Gyeonggi 487-010, Republic of Korea;Division of Animal Science, National Institute of Animal Science, Rural Development Administration, Gyeonggi 441-706, Republic of Korea;Department of Animal Biotechnology, KonKuk University, Seoul 143-701, Republic of Korea

关键词: activation;    ICSI;    IVM;    oocyte;    swine;   

DOI  :  10.1292/jvms.11-0073

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(41)The objective of this study was to evaluate the effect of electrical stimulation (EST) on pronuclear formation, chromosomal constitution, and developmental capability among in vitro matured pig oocytes following intracytoplasmic sperm injection (ICSI). After ICSI, the oocytes were randomly distributed and cultured into 3 groups: the EST activated ICSI group, non-activation ICSI group, and in vitro fertilization (IVF) group. The proportion of oocytes in which 2 pronuclei were formed in ICSI groups was significantly higher in the former groups than in the IVF group (96.2 and 93.5 vs. 64.5%, respectively, PPP<0.05). Diploid blastocysts were observed in 52.4, 63.0, and 65.2% of oocytes in the IVF, activated, and non-activated ICSI groups, respectively. Eight out of 23 gilts (34.8%) were confirmed to be pregnant in activated ICSI groups, but none of these pregnancies were carried to term. These results show that oocyte activation after ICSI is effective in elevating the cleavage rate and blastocyst development, while ensuring normal chromosome composition. Further research is needed to determine the pregnancy maintenance requirements for ICSI-embryos in pigs.

【 授权许可】

Unknown