Ovarian slicing technique to obtain oocytes from agoutis (Dasyprocta prymnolopha)

Pesquisa Veterinária Brasileira

Carvalho, Maria Acelina Martins de¹  Moraes Júnior, Felipe de Jesus²  Pessoa, Gerson T.¹  Feitosa, Matheus Levi T.¹  Albuquerque, Daniel M. de N.¹  Almeida, Hatawa M. de¹  Bezerra, Dayseanny de O.¹  Ferraz, Maíra S.³ 

[1] UFPI, Teresina, Brazil;Universidade Estadual do Maranhão, São Luís, Brazil;Universidade Federal do Piauí, Teresina, Brazil

关键词:  Ovarian slicing technique;    oocytes;    agoutis;    Dasyprocta prymnolopha;    rodent;    cumulus-oocytes complex (COCs);    IVM;   

DOI  :  10.1590/S0100-736X2016000300009

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

:The reproductive biotechnologies are important tools for conservation of domestic and wild animal species, because they allow the recovery and future use of reproductive material. The aim of this study was to evaluate morphological parameters of COCs of agoutis obtained by slicing the ovary for using them in maturation and fertilization protocols in vitro production of embryos. Seventeen female agoutis NEPAS, CCA-UFPI, age and weight average of 3.9 years and 2.16 kg, respectively, were underwent ovariossalpingohisterectomy. The ovaries after dissected and weighed on a precision balance were sliced individually. We proceeded the search and selection of COCs in stereomicroscope, which were identified and quantified by each ovary, and classified as to their morphology, by the quantity of layers of cumulus cells and the cytoplasm into four degrees. It has been found that the technique of slicing ovarian possible to obtain agouti COCs with recovery of loads and varying degrees of quality. There was no correlation between age and weight of animal ovaries, age and the number of COCs obtained; between the weight of agoutis and weight of ovaries and the number of COCs obtained.Index

【 授权许可】

Unknown