期刊论文详细信息
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Detection of shiga toxin producing Escherichia coli isolated from mastitis milk of sheep by qPCR technique
ARTICLE
Hassan Hachim Naser1 
[1] Coll. of Vet. Med. / Univ. of Al-Qadisiyiah
关键词: Escherichia coli;    Shiga toxin;    Real-Time PCR.;   
DOI  :  10.29079/vol15iss2art385
学科分类:土木及结构工程学
来源: College of Veterinary Medicine
PDF
【 摘 要 】

This study was conducted to determine the Shiga toxin producing Escherichia coli isolated from mastitis milk samples of sheep that collected from Diwanyia city by qPCR technique. A total of (60) clinical investigated mastitis milk samples from sheep were subjected to bacterial DNA extraction by using (PrestoTM Mini gDNA Bacteria Kit). The extracted DNA subjected to Real-Time PCR technique (qPCR) for detection of shiga toxin 1 (stx1) gene. Results were revealed that the mastitis sheep are more prevalence for shedding shiga toxin 1 producing Escherichia coli (13.3%) of (8/60) positive samples. In conclusion, the Real-Time PCR technique was shown high specific and rapid method in detection of shiga toxin gene and the sheep which infected by mastitis can be carried the shiga toxin producing Escherichia coli, that may be considered to be risk factor of human infections.

【 授权许可】

CC BY-NC   

【 预 览 】
附件列表
Files Size Format View
RO202106100002949ZK.pdf 432KB PDF download
  文献评价指标  
  下载次数:1次 浏览次数:0次