Cytotoxic Activity and Apoptosis Induction of Ethanolic Extract of Pericarps of Mangosteen (Garcinia mangostana Linn.) on WiDr Cells and Interaction Study of Alpha-mangosteen to IKK and VEGF Based on Molecular Docking

ISCC (Indonesian Journal of Cancer Chemoprevention)

ARTICLE

Annishfia Lailatur Rohmah¹  Fikri Amalia¹  Erlina Rivanti¹  Dyaningtyas Dewi Pamungkas Putri¹  Nunuk Aries Nurulita¹ 

[1] Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada

关键词: Garcinia mangostana Linn .;    cytotoxic effect;    apoptosis;    WiDr cell;    molecular docking;   

DOI  :  10.14499/indonesianjcanchemoprev4iss1pp470-476

学科分类：药学

来源: Indonesian Society for Cancer Chemoprevention

【 摘 要 】

One of the compounds found efficacious as an anti-proliferative on colon cancer is alpha-mangosteen, a xanthon compound that is abundant in pericarp of mangosteen. This study focused to evaluate anticancer activity of the ethanolic extract of pericarp of mangosteen ( Garcinia mangostana Linn.) on WiDr colon cancer cells and to observe the affinity of alpha-mangosteen in inhibiting IKK and VEGF. Cytotoxic effect was tested by MTT assay and apoptosis induction was evaluated by double staining method on WiDr colon cancer cells, while interaction between alpha-mangosteen to the receptors was measured by molecular docking. The ethanolic extract was proven to have cytotoxic activity against WiDr colon cancer cells with IC 50 of 25 µg/mL. In addition, the observation of apoptosis induction by double-staining method showed that apoptosis associated the cytotoxic effect of ethanolic extract of pericarp of mangosteen (EPM) on WiDr colon cancer cells. Molecular docking showed that active compounds in pericarp of mangosteen could compete with the native ligand of VEGF because the docking score of alpha-mangosteen was almost equal with native ligand. From these results we could be concluded that the cytotoxic effect of EPM to WiDr cells was mediated by cell apoptosis. This extract was potential to be developed as chemopreventive agent.

【 授权许可】

CC BY-NC