Evaluating Chondroitin Sulfate and Dermatan Sulfate Expression in Glial Scar to Determine Appropriate Intervention Time in Rats

Basic and Clinical Neuroscience

ARTICLE

Rezaei, Sara¹  Bakhtiyari, Salar²  Assadollahi, Khairolah³  Heidarizadi, Somayeh¹  Moayeri, Ardashir¹  Azizi, Monireh¹ 

[1] Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences;Department of Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences;Department of Epidemiology, Faculty of Health, Ilam University of Medical Sciences;Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences

关键词: Spinal cord injury;    Glial scar;    Chondroitin sulfate;    Dermatan sulfate;   

DOI  :  10.32598/bcn.9.10.405

来源: Iran University of Medical Sciences

【 摘 要 】

Introduction: The proteoglycans of the extracellular matrix increases in the glial scar during spinal cord injury and significantly affects the inhibition of axonal regeneration. Methods: The results of injury therapies are limited due to the lack of identifying a timely therapeutic intervention. The present study aimed to investigate the glial scar Chondroitin Sulfate (CS) and Dermatan Sulfate (DS) levels at different post-injury times to determine the appropriate time for therapeutic intervention. Results: By this experimental study, 72 Wistar rats were randomly divided into 12 groups, as follows: control, sham, injured animals at 1, 2, 4, and 8 days, as well as 2, 4, 8, 12, 16, and 20 weeks post-injury. The animals in the injured groups were contused in the T10 segment of the spinal cord. The motor function of animals was assessed using the Basso, Beattie, and Bresnahan (BBB) test. Besides, the histological assessment was performed using Luxol Fast Blue and Bielshovisky Staining. The CS and DS levels of lesions were measured using the Enzyme-Linked Immunosorbent Assay (ELISA) method. Conclusion: The motor function assessment indicated a relative recovery over time. Histological results confirmed some regeneration in the injury site at 20 weeks post-injury. The ELISA results demonstrated a much higher level of DS than that of CS in the glial scar. Considering high levels of DS, compared to CS in the glial scar and its reduction from second weeks after SCI onwards, the second week after SCI seems to be the best time for therapeutic interventions in terms of scar permeability.

【 授权许可】

Unknown