Molecular Analysis of rs2070744 and rs1799983 Polymorphisms of NOS3 Gene in Iranian Patients With Multiple Sclerosis

Basic and Clinical Neuroscience

ARTICLE

Heidari, Mohammad Mehdi¹  Khatami, Mehri¹  Tahamtan, Yaser² 

[1] Department of Biology, Faculty of Science, Yazd University;Department of Biology, Ashkezar Branch, Islamic Azad University

关键词: Multiple sclerosis;    NOS3 gene;    rs2070744;    rs1799983;    Polymorphism;   

DOI  :  10.18869/nirp.bcn.8.4.279

来源: Iran University of Medical Sciences

【 摘 要 】

Introduction: Multiple Sclerosis (MS) is a disease of central nervous system that mainly causes lesions or plaques in the spinal cord and brain. The purpose of this study was to analyze the relation between c.-813C>T (rs2070744) and c.894G>T (rs1799983) polymorphisms of NOS3 gene and MS in Iranian patients. Methods: A total of 78 patients with MS and 80 healthy controls were screened for NOS3 (rs2070744 and rs1799983) Single Nucleotide Polymorphisms (SNPs) by tetra-primer multiplex ARMS-PCR and PCR-RFLP. Results: Genotype frequencies of the c.-813C>T polymorphism in patients compared to controls were as follows: 53.8% to 80.0% for TT genotype, 41.0% to 18.8% for TC genotype, and 5.1% versus 1.2% for CC genotype (P=0.001). The frequencies of GG genotype was 57.7% and 78.8% and for GT genotype of c.894G>T polymorphism in patients compared to control subjects was 42.3% and 21.2%, respectively (P=0.004). Conclusion: Our results indicate that the studied NOS3 polymorphisms may be associated with MS in Iranian patients.

【 授权许可】

Unknown