Evaluation of Asarum europaeum L. Rhizome for the Biological Activities Related to Alzheimer’s Disease

Research Journal of Pharmacognosy

ARTICLE

Mina Saeedi¹  Yasaman Vahedi-Mazdabadi²  Arezoo Rastegari²  Mahdieh Soleimani³  Mahdieh Eftekhari⁴  Tahmineh Akbarzadeh²  Mahnaz Khanavi² 

[1] Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences;Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences;School of Pharmacy, International Campus (TUMS-IC), Tehran University of Medical Sciences;Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences;Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences;Faculty of Land and Food Systems, University of British Columbia

关键词: Alzheimer’s disease;    Asarum europaeum;    cholinesterase inhibitors;    neuroprotection;    traditional medicine;   

DOI  :  10.22127/rjp.2020.223143.1565

学科分类：环境科学（综合）

来源: Iranian Society of Pharmacognosy

【 摘 要 】

Background and objectives: Asarum europaeum L. is an herbal medicine belonging to the family Aristolochiaceae. The rhizome of the plant has been used for the treatment of various diseases in complementary and alternative medicine of various countries. In Iranian traditional medicine (ITM), the aqueous extract of the rhizome has been used for the improvement and enhancement of memory. Methods: In the present study, the aqueous and hydroalcoholic extracts as well as different fractions of A. europaeum rhizome were evaluated for their cholinesterase (ChE), acetyl- and butyrylcholinesterase (AChE and BChE) inhibitory activity via modified Ellman's method. Results: The ethyl acetate fraction selectively showed the most suitable anti-AChE activity (IC50 = 99.69 µg/mL); none of the extracts or fractions demonstrated anti-BChE activity. In this regard, the ethyl acetate fraction was candidate for the investigation of further biological activities such as antioxidant activity, neuroprotectivity, and metal chelating ability related to Alzheimer’s disease. It depicted favorable neuroprotectivity at concentration of 100 μg/mL against the toxicity of exposure to H2O2 in PC12 cells (p0.001, cell viability = 80/60%) and chelating ability towards zinc, iron, and copper ions. The results of antioxidant activity by DPPH assay showed that the ethyl acetate fraction was much more potent than BHA as the reference drug. Conclusion: The ethyl acetate fraction of A. europaeum L. showed potent biological activities involved in Alzheimer’s disease and needs complementary investigations to develop an herbal product against Alzheimer’s disease.

【 授权许可】

CC BY-NC