Asian Dust Particles Induce TGF-β 1 via Reactive Oxygen Species in Bronchial Epithelial Cells

Tuberculosis and Respiratory Diseases

article

Sun Young Kyung¹  Jin Young Yoon¹  Yu Jin Kim¹  Sang Pyo Lee¹  Jeong-Woong Park¹  Sung Hwan Jeong¹ 

[1] Division of Pulmonology, Department of Internal Medicine, Gachon University Gil Hospital, Gachon University of Medicine and Science, Incheon

关键词: Air Pollutants;    Reactive Oxygen Species;    Transforming Growth Factor β;   

DOI  :  10.4046/trd.2012.73.2.84

学科分类：医学（综合）

来源: The Korean Academy of Tuberculosis and Respiratory Diseases

【 摘 要 】

Background Asian dust storms can be transported across eastern Asia. In vitro , Asian dust particle-induced inflammation and enhancement of the allergic reaction have been observed. However, the fibrotic effects of Asian dust particles are not clear. Production of transforming growth factor β 1 (TGF-β 1 ) and fibronectin were investigated in the bronchial epithelial cells after exposure to Asian dust particulate matter (AD-PM10). Methods During Asian dust storm periods, air samples were collected. The bronchial epithelial cells were exposed to AD-PM10 with and without the antioxidant, N-acetyl-L-cysteine (NAC). Then TGF-β 1 and fibronectin were detected by Western blotting. The reactive oxygen species (ROS) was detected by the measurement of dicholorodihydrofluorescin (DCF), using a FACScan, and visualized by a confocal microscopy. Results The expression of TGF-β 1 , fibronectin and ROS was high after being exposed to AD-PM10, compared to the control. NAC attenuated both TGF-β 1 and fibronectin expression in the AD-PM10-exposed the bronchial epithelial cells. Conclusion AD-PM10 may have fibrotic potential in the bronchial epithelial cells and the possible mechanism is AD-PM10-induced intracellular ROS.

【 授权许可】

CC BY-NC