Cyclic AMP downregulates c‐myc expression by inhibition of transcript initiation in human B‐precursor Reh cells

FEBS Letters

Taskén, Kjetil¹  Blomhoff, Heidi Kill²  Andersson, Kristin Brevik² 

[1] University of Oslo, Institute of Medical Biochemistry, PB 1112, N-0317 Oslo, Norway;The Norwegian Radium Hospital, Institute for Cancer Research, Department of Immunology, Montebello, N-0310, Norway

关键词: c-myc;    Transcriptional inhibition;    cAMP;    B lymphocyte;    cAMP;    cyclic AMP;    adenosine 3':5'-cyclic monophosphate;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    TFGβ;    Transforming Growth Factor β;   

DOI  :  10.1016/0014-5793(94)80632-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the human pre-B cell line Reh, activation of the cyclic AMP signal transduction pathway induces a rapid, transient 10-fold down-regulation of steady-state c-myc mRNA. We have investigated the mechanisms involved in this cAMP-mediated regulation of c-myc expression. Forskolin did not alter c-myc mRNA stability. Initiation of c-myc transcripts was strongly inhibited after 1 h of forskolin treatment, as measured by nuclear run-on assays. Reinitiation of c-myc transcription was apparent after 3–4 h, and full transcriptional elongation was detected after 8 h of forskolin treatment. These data suggest that cyclic AMP downregulates c-myc expression by inhibition of transcriptional initiation.

【 授权许可】

Unknown