The Effect of Post-Treatment N -Acetylcysteine in LPS-Induced Acute Lung Injury of Rats

Tuberculosis and Respiratory Diseases

article

Jae Sung Choi¹  Ho Sung Lee¹  Ki Hyun Seo¹  Ju Ock Na¹  Yong Hoon Kim¹  Soo Taek Uh¹  Choon Sik Park¹  Mee Hye Oh²  Sang Han Lee³  Young Tong Kim⁴ 

[1] Department of Internal Medicine, Clinical Research Institute, Soonchunhyang University College of Medicine;Department of Pathology, Clinical Research Institute, Soonchunhyang University College of Medicine;Department of Biochemistry, Clinical Research Institute, Soonchunhyang University College of Medicine;Department of Radiology, Clinical Research Institute, Soonchunhyang University College of Medicine

关键词: Acetylcysteine;    Acute Lung Injury;    Antioxidants;   

DOI  :  10.4046/trd.2012.73.1.22

学科分类：医学（综合）

来源: The Korean Academy of Tuberculosis and Respiratory Diseases

【 摘 要 】

Background Oxidation plays an important role in acute lung injury. This study was conducted in order to elucidate the effect of repetitive post-treatment of N -acetylcysteine (NAC) in lipopolysaccaride (LPS)-induced acute lung injury (ALI) of rats. Methods Six-week-old male Sprague-Dawley rats were divided into 4 groups. LPS ( Escherichia coli 5 mg/kg) was administered intravenously via the tail vein. NAC (20 mg/kg) was injected intraperitoneally 3, 6, and 12 hours after LPS injection. Broncho-alveolar lavage fluid (BALF) and lung tissues were obtained to evaluate the ALI at 24 hours after LPS injection. The concentration of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) were measured in BALF. Nuclear factor κB (NF-κB), lipid peroxidation (LPO), and myeloperoxidase (MPO) were measured using lung tissues. Micro-computed tomography (micro-CT) images were examined in each group at 72 hours apart from the main experiments in order to observe the delayed effects of NAC. Results TNF-α and IL-1β concentration in BALF were not different between LPS and NAC treatment groups. The concentration of LPO in NAC treatment group was significantly lower than that of LPS group (5.5±2.8 nmol/mL vs. 16.5±1.6 nmol/mL) (p=0.001). The activity of MPO in NAC treatment group was significantly lower than that of LPS group (6.4±1.8 unit/g vs. 11.2±6.3 unit/g, tissue) (p<0.048). The concentration of NF-κB in NAC treatment group was significantly lower than that of LPS group (0.3±0.1 ng/µL vs. 0.4±0.2 ng/µL) (p=0.0001). Micro-CT showed less extent of lung injury in NAC treatment than LPS group. Conclusion After induction of ALI with lipopolysaccharide, the therapeutic administration of NAC partially attenuated the extent of ALI through the inhibition of NF-κB activation.

【 授权许可】

CC BY-NC