An Efficient DNA Extraction Method for Lactobacillus casei , a Difficult-to-Lyse Bacterium

International Journal of Enteric Pathogens

article

Mojtaba Alimolaei¹  Mehdi Golchin¹ 

[1] Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman;Department of Anaerobic Bacterial Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Kerman Branch

关键词: DNA;    Extraction;    Modified Protocols;    Lactobacillus casei;   

DOI  :  10.17795/ijep32472

学科分类：农业科学（综合）

来源: Alborz University of Medical Sciences

【 摘 要 】

Background: There are several protocols to extract DNA from Lactobacillus spp. In the case of L. casei it is harder because of its especial and thick cell wall. Objectives: In this study, nine DNA extraction protocols (by lysozyme treatment) were evaluated and compared in two categories (traditional and kit-based protocols) and an improved method was presented. Materials and Methods: DNA quantity and quality was determined by spectrophotometry, agarose gel electrophoresis and polymerase chain reaction (PCR). Results: The results revealed that the yield of extracted DNA differed by each protocol (5.8 - 17.1 μg/100 μL), but provided appropriate DNA for PCR amplification. The modified protocol offered the best total DNA extraction method when both quality (DNA purity; 1.54 μg) and quantity (DNA yield; 17.1 μg) were considered. Conclusions: We suggest this protocol for effective and inexpensive DNA isolation from L. casei for downstream biological processes such as PCR.

【 授权许可】

CC BY-NC